Ang II诱导大鼠心肌细胞肥大过程中分泌型卷曲相关蛋白5表达上调

基础医学与临床 ›› 2018, Vol. 38 ›› Issue (1): 20-25.

Ang II诱导大鼠心肌细胞肥大过程中分泌型卷曲相关蛋白5表达上调

金鑫,郭炳彦,李拥军

河北医科大学第二医院

收稿日期:2016-11-17 修回日期:2017-03-24 出版日期:2018-01-05 发布日期:2017-12-26
通讯作者: 李拥军 E-mail:jinxin99123@sohu.com
基金资助:
国家自然科学基金项目

Ang II induces up-expression of secreted frizzled-related protein 5 in cardiomyocyte hypertrophy of rats

Received:2016-11-17 Revised:2017-03-24 Online:2018-01-05 Published:2017-12-26

摘要/Abstract

摘要： 目的探讨分泌型卷曲相关蛋白5（sFRP5）在大鼠心肌细胞肥大中表达的机制。方法体外培养乳鼠心肌细胞，用血管紧张素II（AngII 10-6mmol/L,48 h）诱导心肌细胞肥大。应用替米沙坦和Y27632分别阻断血管紧张素1型受体（AT1R）及Rho/Rho激酶（Rho/ROCK）信号通路；PD98059、SB203580及SP600125分别阻断p38 MAPK、ERK1/2及JNK通路； RT-PCR法检测sFRP5的表达；Western blot法检测sFRP5、ROCK1、ROCK2、总MYPT1、p-MYPT1、总JNK及p-JNK的表达。结果 Ang II诱导的心肌细胞肥大致sFRP5表达上调（P<0.05），Y27632下调sFRP5的表达（P<0.05）；替米沙坦下调ROCK1的表达（P<0.05）；SP600125明显降低sFRP5表达的增加（P<0.05）。结论在Ang II诱导的心肌细胞肥大过程中，主要通过Rho/ROCK1/JNK通路上调sFRP5的表达。

Abstract: Objective To investigate the mechanism of secreted frizzled-related Protein 5 (sFRP5) expression in cardiomyocyte hypertrophy. Methods In vivo experiment, neonatal rat ventricular myocytes were exposed to Ang II（10-6mmol/L, 48h）. Telmisartan, Y27632, PD98059，SB203580 and SP600125 were used to block angiotensin type 1 receptor(AT1R), Rho/ROCK, p38 MAPK, ERK1/2 and JNK pathway, respectively. Western blot was to determine the expressions of sFRP5, ROCK1, ROCK2, total MYPT1, p-MYPT1, total JNK and p-JNK. RT-PCR was used to determine sFRP5 expression. Results Our experiment confirmed that there were significant inhibition of sFRP5 expression when treated with Y37632 and SP699125, but less with SB203580 and PD98059 in Ang II-induced cardiomyocytes. Moreover, telmisartan down-regulated the expression of ROCK1, but had no effect on the expression of ROCK2. Conclusions The expression of sFRP5 was up-regulated mainly by Rho/ROCK1/JNK pathway in cardiomyocyte hypertrophy induced by Ang II.

Key words: Secreted frizzled-related protein 5, Angiotensin II, Cardiomyocyte, hypertrophy, JNK pathway

金鑫郭炳彦李拥军. Ang II诱导大鼠心肌细胞肥大过程中分泌型卷曲相关蛋白5表达上调[J]. 基础医学与临床, 2018, 38(1): 20-25.

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