基础医学与临床 ›› 2017, Vol. 37 ›› Issue (1): 62-66.

• 研究论文 • 上一篇    下一篇

急性髓系白血病患者化疗后微小残留病与多药耐药基因表达相关

李丽君,刘蓓,徐茜,刘欢   

  1. 兰州大学第一医院
  • 收稿日期:2016-07-19 修回日期:2016-10-11 出版日期:2017-01-05 发布日期:2016-12-30
  • 通讯作者: 刘蓓 E-mail:liubeiff@163.com
  • 基金资助:
    甘肃省卫生行业科研计划项目——急性白血病ABC家族多药耐药相关基因的研究;兰州市科技发展计划项目——多参数流式细胞术检测急性髓系白血病微小残留与多药耐药基因表达的相关性研究

Correlation of minimal residual disease and multidrug resistance genes expression in acute myeloid leukemia

  • Received:2016-07-19 Revised:2016-10-11 Online:2017-01-05 Published:2016-12-30

摘要: 目的 明确急性髓系白血病(AML)中微小残留病(MRD)与多药耐药基因表达的相关性。方法 收集初诊AML患者骨髓52例,用real-time PCR检测患者化疗前ABCB1、ABCC1、ABCC4及ABCG2 4种多药耐药基因的表达,同时用多参数流式细胞术(MFC)检测患者诱导化疗后和化疗3、6和9个月后的MRD表达水平。结果 52例AML患者MRD表达水平与ABCB1(P<0.01)、ABCC1(P<0.01)、ABCC4(P<0.01)和ABCG2(P<0.01)的mRNA表达水平具有明显相关性。持续监测9个月后,MRD再次转阳者ABC基因表达水平显著高于持续阴性者:ABCB1(P<0.05)、ABCC1(P<0.05)、ABCC4(P<0.01)和ABCG2(P<0.01)。结论 MRD表达水平与多药耐药基因表达水平显著正相关。

关键词: 急性髓系白血病, 微小残留病, 多药耐药基因, 多参数流式细胞术, 实时荧光定量PCR

Abstract: Objective To explore the correlation of minimal residual disease(MRD) and ABC genes expression in acute myeloid leukemia(AML).Methods 52 de novo AML bone marrow samples were used to detect the expression of ABCB1,ABCC1,ABCC4 andABCG2 by real-time PCR at diagnosis. Meanwhile, followed up these patients to monitor MRD with MFC at the points of finishing the first induction cause as well as the third,the sixth and the ninth month after chemotherapy. Results The expression level of the 4 ABC transporters among three MRD levels detected after induction cause are correlated significantly with ABCB1(P<0.01), ABCC1(P<0.01), ABCC4(P<0.01) and ABCG2(P<0.01).Further more, after nine months follow-up, the patients whose MRD turned into positive again were detected a high ABC transports expression level, comparing with those MRD remained negative for nine months,which was of a statistically significance for ABCB1(P<0.05), ABCC1(P<0.05), ABCC4(P<0.01) and ABCG2(P<0.01). Conclusions There proves a positive correlation of MRD and ABC transporters level in AML.

Key words: acute myeloid leukemia , minimal residual disease, ABC transporters, multi-parameter flow cytometry, real-time PCR

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