基础医学与临床 ›› 2016, Vol. 36 ›› Issue (4): 498-502.

• 研究论文 • 上一篇    下一篇

靶向ANGPTL4基因shRNA慢病毒载体抑制SiHa细胞迁移与侵袭

聂丹1,范凌晔1,刘玲1,詹平2,郑茜文1,毛熙光1   

  1. 1. 泸州医学院附属医院
    2. 四川省泸州医学院附属医院妇产科
  • 收稿日期:2015-10-12 修回日期:2015-12-01 出版日期:2016-04-05 发布日期:2016-03-29
  • 通讯作者: 毛熙光 E-mail:mxg6639@163.com
  • 基金资助:
    四川省卫生厅科研课题;泸州市科技计划项目;泸州医学院青年基金项目

The lentivirus-mediated shRNA targeting ANGPTL4 inhibits the migration and invasion of SiHa cells

  • Received:2015-10-12 Revised:2015-12-01 Online:2016-04-05 Published:2016-03-29

摘要: 目的 探讨慢病毒介导短发夹RNA(shRNA)沉默血管生成素样蛋白4(ANGPTL4)基因对人宫颈癌细胞系SiHa细胞迁移与侵袭的影响。方法 实验分为3组,即空白对照组、阴性对照组及LV3/ANGPTL4组。用ANGPTL4 shRNA慢病毒干扰载体感染SiHa细胞,Western blot检测Integrinβ1、VEGF及MMP9蛋白表达,划痕实验检测SiHa细胞迁移能力,Transwell 实验检测SiHa细胞迁移与侵袭能力。结果 重组慢病毒载体成功感染SiHa细胞,与空白对照组及阴性对照组比较, LV3/ANGPTL4组SiHa细胞Integrinβ1、VEGF及MMP9蛋白表达水平降低(P<0.01),细胞迁移与侵袭能力减弱(P<0.05)。结论 靶向ANGPTL4 基因shRNA慢病毒载体可降低SiHa细胞Integrinβ1、VEGF及MMP9蛋白表达水平,抑制SiHa细胞迁移与侵袭能力。

关键词: 血管生成素样蛋白4, 慢病毒, 迁移, 侵袭

Abstract: Objective To investigate the effect of lentivirus-mediated shRNA silencing angiopoietin-like protein 4(ANGPTL4) gene on migration and invasion of cervical cancer cell line SiHa. Methods In this experiment, three groups were designed: control, negative control and LV3/ANGPTL4 groups. The shRNA for lentiviral interference vectors were infected into SiHa cells. The expressions of Integrinβ1, VEGF and MMP9 protein were detected by Western blot. The migration and invasion ability of SiHa cells were detected by scratch wound assay and Transwell respectively. Results ANGPTL4 shRNA lentiviral interference vectors were successfully infected into SiHa cells. Compared with those in control group and negative control group, the expressions of Integrinβ1, VEGF and MMP9 protein in LV3/ANGPTL4 group were down-regulated(P<0.01). The migration and invasion ability of SiHa cells in LV3/ANGPTL4 group were significantly decreased (P<0.05). Conclusion Lentiviral-shRNA vector targeting ANGPTL4 gene can down-regulate the expression of Integrinβ1, VEGF and MMP9 protein in SiHa cells. It also can effectively inhibit the migration and invasion ability of Siha cells.

Key words: angiopoietin-like protein 4, lentivirus, migration, invasion