基础医学与临床 ›› 2016, Vol. 36 ›› Issue (11): 1548-1553.

• 研究论文 • 上一篇    下一篇

WTAP基因在胃癌组织中低表达

汪毅1,张杰2,皮静楠2,刘晓玲2   

  1. 1. 中国医学科学院 北京协和医学院 肿瘤医院
    2. 中国医学科学院基础医学研究所
  • 收稿日期:2016-08-18 修回日期:2016-09-19 出版日期:2016-11-05 发布日期:2016-10-24
  • 通讯作者: 刘晓玲 E-mail:happyeday@163.com

Expression and functional analysis of WTAP gene in gastric cancer

  • Received:2016-08-18 Revised:2016-09-19 Online:2016-11-05 Published:2016-10-24

摘要: 目的 探讨WTAP基因在胃癌组织样本中的表达变化情况,并检测其对胃癌细胞表型的影响。方法 用qRT-PCR的方法检测45例胃癌组织及其对应的癌旁组织中WTAP mRNA的表达量,并结合临床病理资料进行分析;使用RNA干扰方法敲低内源性WTAP在胃癌细胞系MGC-803中的表达,再通过CCK-8实验、划痕迁移实验和trans-well实验,检测WTAP表达降低后对MGC-803细胞功能的影响。结果 WTAP mRNA在胃癌癌症组织中的表达明显低于癌旁组织(p<0.05),且其表达与胃癌样本的临床分期密切相关(p<0.05);在MGC-803细胞系中敲低WTAP,可促进胃癌细胞增殖、促进细胞迁移和侵袭。结论 WTAP在胃癌组织中低表达,可能与其发生发展有关,有望成为胃癌诊治的靶标。

关键词: 胃癌, WTAP, MGC-803

Abstract: Objective To investigate the expression change of WTAP gene in clinical gastric cancer samples and its functional significance in gastric carcinogenesis. Methods The WTAP mRNA was detected by qRT-PCR in 45 cases of gastric cancer samples and their paired adjacent normal control tissues. The expression of WTAP mRNA was subjected to analysis combined with patients’ clinical pathological data. The effects of WTAP knock-down on cell proliferation, cell migration and invasion were detected by CCK-8, wounding heal and trans-well assays, respectively. Results The expression of WTAP mRNA in gastric tumor tissues was significantly lower than the adjacent tissues (p<0.05), and was closely related with gastric cancer clinical stage (p<0.05). Furthermore, knock-down of WTAP in MGC-803 cells could promote cell proliferation, cell migration and invasion. Conclusions WTAP was down-regulated in gastric cancers, and it might sever as a tumor suppressor gene in gastric carcinogenesis.

Key words: gastric cancer, WTAP, MGC-803

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