基础医学与临床 ›› 2016, Vol. 36 ›› Issue (11): 1467-1471.

• 研究论文 •    下一篇

蓝莓花青素对结肠癌LS174T细胞增殖和凋亡的作用及机制

侯锐,王利,李少伟,贾宇臣,陈琦   

  1. 内蒙古医科大学
  • 收稿日期:2015-08-17 修回日期:2016-01-05 出版日期:2016-11-05 发布日期:2016-10-24
  • 通讯作者: 陈琦 E-mail:yongqi_1@163.com
  • 基金资助:
    自噬与凋亡在蓝莓花青素抗口腔癌作用中的分子机制研究;蓝莓花青素抑制口腔癌KB细胞增殖与p53、p21和p73甲基化相关性研究

The action and mechanism of blueberry anthocyanins on apoptosis and proliferation in human colon cancer cells LS174T

  • Received:2015-08-17 Revised:2016-01-05 Online:2016-11-05 Published:2016-10-24

摘要: 目的 观察花青素对结肠癌LS174T细胞增殖的抑制作用及凋亡的诱导及探究蓝莓花青素的抗癌机制。方法 体外培养结肠癌LS174T细胞,加入不同浓度的蓝莓花青素(25、50、100和 200μg/mL)处理,利用CCK法、Hoechst染色检测不同浓度的蓝莓花青素对LS174T细胞增殖和凋亡的影响;实时荧光定量PCR(real-time PCR)检测细胞相关凋亡基因p53、ccnd3、p73、sfn、caspase8和fam200a mRNA的表达;蛋白质免疫印迹法(Western blot)检测细胞caspase8和p73的表达。结果 蓝莓花青素呈时间和剂量依赖性抑制了结肠癌LS174T的增殖(P<0.05);花青素呈现剂量依赖性诱导癌细胞凋亡; ccnd3、p73和sfn mRNA表达水平上调(P<0.05),caspase8、fam200a和p53 mRNA表达水平下调(P<0.05);caspase8蛋白表达下调,p73蛋白表达上调。结论 蓝莓花青素抑制结肠癌ls174t细胞增殖,诱导凋亡与p73蛋白表达上调和caspase8蛋白表达下调相关。

关键词: 关键词:花青素, 抗癌, 结肠癌, 蓝莓

Abstract: Objective: We observed actions and effects of anthocyanins on antiproliferation and proapoptosis in human colon cancer cells LS174T, making further investigation of anti-cancer mechanism of blueberry anthocyanin, accordingly, those provide the experimental basis for the prevention and therapy of colon cancer in clinic. Methods: Colon cancer cells ls174t were cultured in vitro,treating LS174T cells with blueberry anthocyanins (25、50、100 and 200μg/mL).We tested the effects of blueberry anthocyanins on proliferation and apoptosis by CCK and Hoechst 33258 chromatin staining dye; We analyzed mRNA expression of apoptosis-related genes(including p53、ccnd3、p73、sfn、caspase8 and fam200a) by real-time PCR. In addition, protein levels of caspase8 and p73 were also analyzed using western blot. Results: The blueberry anthocyanins suppressed the proliferation and induced the apoptosis of LS174T cells respectively in a dose-dependent and time-dependent manners; the expression of ccnd3、p73 and sfn mRNA were up-regulated while the expression of caspase8、fam200a and p53 mRNA were down-regulated; the expression of protein levels of caspase8 and p73 were down-regulated and up-regulated respectively. Conclusion: Blueberry anthocyanins induce cell cycle arrest and apoptosis of colon cancer LS174T cells, which were associated with an up-regulation of the expression of tumor suppressor p73 and a down-regulation of the expression of caspase8.

Key words: Key words: anthocyanins, anticancer, colon cancer, blueberry

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