基础医学与临床 ›› 2016, Vol. 36 ›› Issue (1): 53-57.

• 研究论文 • 上一篇    下一篇

水通道蛋白1、5在大鼠急性肺损伤组织中的表达

陈国兵,连珠兰,占珠琴,吴谨准,白海涛   

  1. 厦门大学附属第一医院
  • 收稿日期:2015-05-25 修回日期:2015-10-29 出版日期:2016-01-05 发布日期:2015-12-29
  • 通讯作者: 吴谨准 E-mail:1923731201@qq.com
  • 基金资助:
    福建省医学创新课题基金资助项目

The expression of aquaporin 1 and 5 in the LPS-induced lung injury in rats

  • Received:2015-05-25 Revised:2015-10-29 Online:2016-01-05 Published:2015-12-29

摘要: 目的 探讨水通道蛋白1、5(AQP1、AQP5)在脂多糖(LPS)诱导大鼠急性肺损伤(ALI)组织中的表达变化及意义。方法 将Wistar大鼠48只随机分为对照组(NC)和急性肺损伤(ALI)组,ALI组通过尾静脉注射脂多糖(LPS),分别于2、6、12和24h采集标本。HE染色观察肺组织病理;测定肺湿/干重比(W/D)、肺通透指数;ELISA检测血清肿瘤坏死因子α( TNF-α) 、巨噬细胞炎性蛋白1α(MIP-1α)水平;Western blot、免疫组化和Real- Time PCR法检测肺AQP1、AQP5蛋白和mRNA变化。结果 与对照组比较,ALI组TNF-α、MIP-1α均有显著性升高(P<0.05),至24h点逐渐恢复。注射LPS后2h出现肺泡和间质水肿,炎性细胞浸润,以12h最明显;肺W/D比、肺泡灌洗(BALF)蛋白含量、肺通透指数均较对照组明显升高(P<0.05),12h点达峰值。ALI组各时间点肺AQP1及AQP5 mRNA及蛋白表达均较对照组明显下调(P<0.01),以12h点下降最明显。肺AQP1、AQP5 mRNA表达与肺W/D比、血清TNF-α,MIP-1α存在明显负相关。结论 脂多糖诱导大鼠急性肺损伤,其肺水肿的形成可能与AQP1、AQP5的表达下调有关。

关键词: 水通道蛋白, 肺损伤, 脂多糖, 大鼠

Abstract: Objective To investigate the expression and significance of the Aquaporin (AQP) 1, 5 in the LPS-induced lung injury in rats. Methods 48 Wistar rats were divided into control-group, ALI-group. Preparation with acute lung injury (ALI) model by intravenous injection of LPS in rats, control-group injected saline. Rats were sacrificed in 2, 6, 12 and 24h, and the samples were collected after the successful modeling. Observing lung HE staining morphological, determining lung wet/dry weight ratio (W/D) and lung permeability index, ELISA detecting serum TNF alpha, MIP-1 alpha level. At the same time, analysis AQP1, AQP5 protein and mRNA content changes through Western-blot, Immunohistochemistry and Real-Time PCR method. Results Compared with control-group, the TNF alpha, MIP-1 alpha level in ALI-group were significantly elevated at 2, 6 and 12h(P<0.05), and to 24h point gradually down to normal level. HE staining showed that the alveolar and interstitial edema and inflammatory cell infiltration after LPS injection 2h, most obviously in 12h. The lung W/D, BALF protein and pulmonary permeability index in ALI-group were significantly higher than those of the control-group (P<0.05), and reached the peak in 12h. At the same time, the expression levels of AQP1, AQP5 in ALI-group were significantly lower than that in control-group (P<0.01). The correlation analysis showed that the expression of AQP1 and AQP5 mRNA was negatively correlated with the lung W/D ratio, serum TNF alpha and MIP-1 alpha level. Conclusion LPS can induces rats acute lung injury, The formation of pulmonary edema may be related to the down-regulation of AQP1 and AQP5.

Key words: Aquaporins, Lung injury, Lipopolysaccharide, Rats