基础医学与临床 ›› 2015, Vol. 35 ›› Issue (9): 1176-1181.

• 研究论文 • 上一篇    下一篇

H3K27me3去甲基化酶JMJD3调节胎鼠肺上皮细胞增殖和分化

冯欢,张超,张哲恺,章铭,张丰   

  1. 第四军医大学
  • 收稿日期:2015-01-19 修回日期:2015-04-25 出版日期:2015-09-05 发布日期:2015-09-07
  • 通讯作者: 张丰 E-mail:zhf1975@fmmu.edu.cn

H3K27me3 demethylase JMJD3 regulates proliferation and differentiation of embryonal lung epithelia of mice

  • Received:2015-01-19 Revised:2015-04-25 Online:2015-09-05 Published:2015-09-07
  • Contact: Feng Zhang E-mail:zhf1975@fmmu.edu.cn

摘要: 目的 分析H3K27me3特异去甲基化酶JMJD3(KDM6B)在胎鼠肺生长发育过程中的作用。方法 HE染色、PAS染色和免疫组化方法检测E19.5JMJD3基因敲除胎鼠肺生长发育情况。结果 与野生型(Jmjd3+/+)胚胎比较,杂合型敲除(Jmjd3+/-)胚胎的肺发育稍差,但纯合型敲除(Jmjd3-/-)胚胎肺发育明显不良,支气管的纤毛上皮细胞和Clara 细胞以及肺泡的I型和II型上皮细胞分化不良。Jmjd3-/-胚胎肺上皮细胞增殖指数高于野生型和Jmjd3+/-组,未发现Jmjd3-/-胚胎肺上皮细胞凋亡异常。结论JMJD3对胎鼠肺上皮细胞的增殖和分化具有重要调节作用。

关键词: JMJD3, 肺上皮细胞, 增殖, 分化

Abstract: Objective To investigate the role H3K27me3 demethylase JMJD3(KDM6B) during the development of lung in embryonal mice. Methods Jmjd3 knockout embryos of E19.5 mice was examined by HE, PAS and immnohistochemistry assays. Results The developmental defects of the lung of Jmjd3 heterozygous (Jmjd3+/?) embryos were mild compared to Jmjd3+/+ embryos. However, Jmjd3?/? mice suffered from the severe hypoplasia of lung tissue. Differentiated defects of ciliated cell, Clara cell, type I and II alveolar epithelial cells were observed in Jmjd3?/? embryos. The index of cell proliferation was increased in Jmjd3?/? embryos compared to wildtype and Jmjd3+/? embryos. No difference of apoptosis was detected in these embryos. Conclusion JMJD3 is indispensable for proliferation and differentiation of embryonal lung epithelia of mice.

Key words: JMJD3, epithelia of lung, proliferation, differentiation