基础医学与临床 ›› 2015, Vol. 35 ›› Issue (5): 668-673.

• 研究论文 • 上一篇    下一篇

5-羟色胺及其受体亚型在慢性前列腺炎大鼠脊髓的差异性表达

张述蓉1,赵秀娟1,李露2,徐晨1   

  1. 1. 重庆医科大学
    2. 重庆医科大学生命科学研究院
  • 收稿日期:2014-12-25 修回日期:2015-03-13 出版日期:2015-05-05 发布日期:2015-04-28
  • 通讯作者: 徐晨 E-mail:xuchen@cqmu.edu.cn
  • 基金资助:
    重庆市自然科学基金重点项目

Differential expressions of serotonin and its receptor subtypes in spinal cord of chronic prostatitis model in rat

  • Received:2014-12-25 Revised:2015-03-13 Online:2015-05-05 Published:2015-04-28

摘要: 目的 研究5-HT和5-HT2A、5-HT2C、5-HT5A和5-HTR7受体(5-HTR2A、5-HTR2C、5-HTR5A和5-HTR7)在慢性前列腺炎/慢性盆腔疼痛综合征(CP/CPPS)大鼠T13~S1脊髓节段的差异性表达情况。方法 将SD大鼠随机分为对照组、45、60和90d模型组,用自身免疫法构建CP/CPPS模型。用免疫组织化学及RT-PCR法检测脊髓T13~L2、L3~L4和L5~S1节段5-HT及各受体的表达。结果 CP/CPPS大鼠自发性疼痛行为学评分显著高于对照组(P<0.05)。5-HT在各模型组大鼠脊髓3个节段表达均下降。在T13~L2节段, 5-HTR2A、5-HTR5A的表达持续上调(P<0.05),5-HT2C、5-HTR7先升高后下降(P<0.05);在L3~L4节段各受体表达均先升高后下降(P<0.05);在L5~S1节段,5-HT2C、5-HTR5A的表达持续上调(P<0.05),5-HTR2A、5-HTR7先升高后下降(P<0.05)。结论 5-HT及5-HTR2A、5-HTR2C、5-HTR5A和5-HTR7在不同时程CP/CPPS大鼠脊髓出现差异性表达,提示5-HT系统参与了CP/CPPS的中枢调控,其具体机制有待进一步的研究。

关键词: 5-羟色胺, 5-羟色胺受体, 慢性前列腺炎, 脊髓, 大鼠

Abstract: Objective To study of the different expression of serotonin(5-HT) and serotonin receptor subtypes(5-HTRs) in T13~S1 spinal segments of chronic prostatitis rat models. Methods Sprague Dawley rats were randomly divided into control group,45,60 and 90 d model groups. Chronic prostatitis /chronic pelvic pain syndrome of rats were induced by autoimmune. The level of 5-HT was detected by Immunohistochemistry and the expression of 5-HTRs gene were detected by RT-PCR in T13~L2,L3~L4,L5~S1spinal segments in chronic prostatitis rats. Results The spontaneous pain behavior score of CP/CPPS rats was significantly higher compared with control group (P <0.05). The level of 5-HT in three spinal segments in all CP/CPPS model groups were decreased, 45d and 90d model group decreased significantly (P<0.05). The expression of 5-HTR2A,5-HTR5A showed a gradually increasing and 5-HT2C,5-HTR7 were increased at the first and then decreased in T13~L2 spinal segments (P<0.05). The expression of all receptors were decline after rising first in L3~L4 spinal segments (P<0.05). The expression of 5-HT2C,5-HTR5A showed a gradually increasing and 5-HTR2A,5-HTR7 were decline after rising first in L5~S1 spinal segments (P<0.05). Conclusion Our data indicated that there is a distinct expression pattern of 5-HT and 5-HTRs at different stages of CP/CPPS rat, suggesting that 5-HT system may involved in the central regulation mechanism of CP/CPPS,further study are needed for the specific mechanism.

Key words: serotonin, serotonin receptors, chronic prostatitis, spinal cord, rat

中图分类号: