基础医学与临床 ›› 2015, Vol. 35 ›› Issue (3): 345-349.

• 研究论文 • 上一篇    下一篇

XAV939抑制人肝癌HepG2细胞血管生成拟态的形成

汤晓颖1,庞林宾2,宋立文3,王洪林1   

  1. 1. 重庆医科大学附属第一医院
    2. 山东省德州市人民医院
    3. 重庆医科大学附属大学城医院
  • 收稿日期:2014-09-28 修回日期:2014-11-28 出版日期:2015-03-05 发布日期:2015-03-03
  • 通讯作者: 王洪林 E-mail:whlcqykd@163.com

XAV939 inhibits vasculogenic mimicry formation in human hepatoma hepG2 cells

  • Received:2014-09-28 Revised:2014-11-28 Online:2015-03-05 Published:2015-03-03

摘要: 目的 初步研究XAV939对人肝癌HepG2细胞血管生成拟态形成的影响及其可能的机制。方法 实验分为对照组和实验组(0.5和1μmol/L XAV939分别处理HepG2细胞48h);体外成管实验检测各组细胞形成血管拟态的能力,RT-PCR检测ZEB1及MMP-7 mRNA的表达,Western blot检测β-catenin、ZEB1和MMP-7蛋白的表达。结果 对照组与实验组形成管状结构数目分别为9.67±0.70,5.67±0.64(0.5μmol/L)和 2.27±0.81(1μmol/L),体外形成管道结构的数目减少(P<0.01);实验组ZEB1及MMP-7 mRNA表达和ZEB1、MMP-7及β-catenin蛋白表达均较对照组减少(P<0.05)。结论 XAV939能有效抑制人肝癌HepG2细胞血管生成拟态的形成。

关键词: XAV939, HepG2, Wnt/β-catenin, 血管生成拟态

Abstract: Objective To investigate the impact of XAV939 to human hepatoma HepG2 cells vascular mimicry and its possible mechanism. Methods HepG2 cells were divided into control group and XAV939 treatment groups with dose of 0.5 and 1μmol/L; Tube formation of tumor cells experiment to detect the forming tubes ability of each group , RT-PCR were used to detect expressed ZEB1 and MMP-7 mRNA levels , Western blot were used to detect expression of β-catenin, ZEB1 and MMP-7 on protein levels. Results The number of vascular mimicry in the control group and the experimental group. In the control group : 9.67 ± 0.70 , 0.5μmol/L group : 5.67 ± 0.64 , 1μmol/L group : 2.27 ± 0.81,which means the number of pipeline structure formation reducing in vitro (P<0.01). Experimental group compared with the control group, ZEB1 and MMP-7 mRNA expression reduce; ZEB1, MMP-7 and β-catenin protein expression decreased(P<0.05). Conclusion XAV939 can effectively inhibition of vasculogenic mimicry formation in human hepatoma HepG2 cells.

Key words: XAV939, HepG2 , Wnt/β-catenin , vascular mimicry

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