基础医学与临床 ›› 2015, Vol. 35 ›› Issue (3): 341-344.

• 研究论文 • 上一篇    下一篇

db/db小鼠心、肾RAS组分的分子表达

杨久琳,刁腾月,张岩   

  1. 上海理工大学
  • 收稿日期:2014-08-25 修回日期:2014-11-29 出版日期:2015-03-05 发布日期:2015-03-03
  • 通讯作者: 张岩 E-mail:medicineyan@aliyun.com

Molecular expression of RAS components in heart and kidney of db/db mice

  • Received:2014-08-25 Revised:2014-11-29 Online:2015-03-05 Published:2015-03-03

摘要: 目的 探讨2型糖尿病诱导心脏、肾脏肾素-血管紧张素系统(RAS)组分的表达变化。方法 用db/db小鼠作为2型糖尿病动物模型,RT-PCR和蛋白免疫印迹法分别检测心脏、肾脏中RAS组分的基因和蛋白表达。结果 db/db小鼠心脏AGT mRNA表达、Renin和Ang II蛋白表达显著升高(P < 0.01);而肾脏Renin mRNA(P < 0.001)和蛋白(P < 0.01)表达以及Ang II(P < 0.05)的蛋白表达显著上调。结论 2型糖尿病诱导心脏、肾脏RAS组分的异常表达,最终引起RAS活性肽Ang II生成增加。

关键词: 肾素-血管紧张素系统, 心脏, 肾脏, 2型糖尿病

Abstract: Objective To investigate the type 2 diabetes-induced expression changes of renin-angiotensin system (RAS) components in heart and kidney. Methods The db/db mice were applied to be the type 2 diabetes animal model. Gene and protein expression of RAS components in heart and kidney were measured by RT-PCR and immunoblotting, respectively. Results The mRNA expression of AGT (P < 0.01) and the protein expression of Renin (P < 0.01) and Ang II (P < 0.01) were significantly increased in heart of db/db mice, moreover, the mRNA (P < 0.001) and protein (P < 0.01) expression of Renin and the protein expression of Ang II (P < 0.05) were significantly up-regulated in kidney of db/db mice. Conclusion Type 2 diabetes induced the abnormal expression of RAS components in heart and kidney, consequently, leading to the increase of production of RAS active peptide Ang II.

Key words: renin-angiotensin system, heart, kidney, type 2 diabetes