基础医学与临床 ›› 2015, Vol. 35 ›› Issue (3): 318-322.

• 研究论文 • 上一篇    下一篇

褪黑素减轻浸水-束缚应激大鼠胃黏膜损伤

桂红珍1,杨浪1,金鹏2,盛剑秋2   

  1. 1. 北京军区总医院
    2. 北京军区总医院消化内科
  • 收稿日期:2014-09-18 修回日期:2014-12-01 出版日期:2015-03-05 发布日期:2015-03-03
  • 通讯作者: 桂红珍 E-mail:yangzs70@qq.com
  • 基金资助:
    全军医学科技“十二五”科研项目

Melatorin alleviates gastric mucosal injury induced by water-immersion and restraint stress in rats

  • Received:2014-09-18 Revised:2014-12-01 Online:2015-03-05 Published:2015-03-03
  • Contact: Hong-Zhen GUI E-mail:yangzs70@qq.com

摘要: 目的 探讨褪黑素(MT)对大鼠应激性胃溃疡的保护作用及对胃黏膜MMP9、MMP2 和TIMP-2表达的影响。方法 用浸水-束缚应激(WIRS) 建立大鼠应激性溃疡模型。应激前30 min,分别腹腔注射褪黑素10、20、40和60mg/kg。应激3h后,观察大鼠胃黏膜病变,对溃疡指数(UI)进行评分;组织学观察胃出血;用Real-Time PCR及Western blot检测胃黏膜MMP9、MMP2 和TIMP-2的mRNA及蛋白表达。结果 WIRS应激3h能成功诱导大鼠应激性胃溃疡。预先注射MT可明显降低应激性胃溃疡大鼠的UI(P < 0. 01),明显降低黏膜层炎性细胞浸润数(P < 0. 01)。WIRS诱导大鼠胃黏膜MMP9和MMP2 mRNA和蛋白表达上调(P < 0. 01),TIMP-2则下降;MT预处理后,MMP-2/MMP-9明显下降(P < 0. 05或P < 0. 01); 而TIMP-2的mRNA及蛋白表达增加(P < 0. 05或P < 0. 01)。结论 MT可能通过下调MMP9和MMP2表达,及增加TIMP-2表达,减轻WIRS诱导的大鼠应激性胃溃疡。

关键词: 应激, 胃溃疡, 褪黑素, 基质金属蛋白酶, 细胞外基质

Abstract: Objective To investigative the protective effects of melatonin (MT) in gastric stress ulcer of rat and expression of MMP9, MMP2 and TIMP-2 in mucosa. Methods Stress ulcer model was established by water-immersion and restraint stress (WIRS). Melatonin at dose of 10、20、40、60mg/kg were administrated in different group of rat at 30 min before the onset of WIRS. The rat mucosa was examined and ulcer index were evaluated after 3 hours stress. Mucosa hemorrhage was determined at histology. In addition, the mRNA and protein level of MMP9、MMP2 and TIMP-2 was measured by PCR and western blot. Results The rat stress ulcer model can be established by WIRS at 3 hours. Pre-administration of melatonin can decrease the ulcer index (UI) and inflammatory cells in mucosa(P < 0. 01). In addition, the mRNA and protein level of MMP9 and MMP2 was increased, TIMP-2 was decreased in WIRS induced rat ulcer model (P < 0.01). Moreover, the mRNA and protein level of MMP2 and MMP9 was inhibited, while TIMP-2 was promoted by melatonin in stress induced ulcer(P < 0. 05 or P < 0. 01). Conclusions These results reveal the alleviation role of Melatonin on WIRS-induced gastric mucosal injury in rats, which may partly through regulating the expression of MMP2, MMP9 and TIMP2.

Key words: stress, gastric ulcer, melatonin, MMPs, extracellular matrix

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