基础医学与临床 ›› 2015, Vol. 35 ›› Issue (3): 308-312.

• 研究论文 • 上一篇    下一篇

宁夏回族AS患者外周血LCAT基因DNA甲基化与血脂水平的关系

陈久凯1,周龙霞1,王艳华1,马文斌1,赵丽1,刘现梅1,杨晓玲1,姜怡邓2   

  1. 1. 宁夏医科大学
    2. 宁夏医学院基础学院病理生理学教研室
  • 收稿日期:2014-10-13 修回日期:2014-11-28 出版日期:2015-03-05 发布日期:2015-03-03
  • 通讯作者: 姜怡邓 E-mail:jwcjyd@163.com
  • 基金资助:
    国家自然科学基金地区项目;宁夏自然科学基金

Relationship between LCAT DNA methylation and serum lipips level in Hui patients with atherosclerosis in Ningxia

  • Received:2014-10-13 Revised:2014-11-28 Online:2015-03-05 Published:2015-03-03

摘要: 目的 探讨宁夏回族动脉粥样硬化(AS)患者外周血中卵磷脂胆固醇酰基转移酶(LCAT)基因启动子区甲基化水平及与血脂的关系。方法 选择经颈部多普勒超声确诊的AS患者35例及正常者42例作为对照组,采用实时荧光定量PCR(qRT-PCR)检测外周血LCAT mRNA的表达,巢式降落式甲基化特异性PCR(nt-MSP)检测外周血LCAT基因DNA启动子区甲基化程度,全自动生化分析仪检测血脂水平。结果 AS组血清TC、TG和LDL水平显著高于对照组(P<0.05);HDL水平低于对照组;与对照组相比,AS组外周血LCAT mRNA表达降低了61.2%(P<0.01),同时LCAT基因甲基化程度升高了1.3倍(P<0.05)。结论 回族AS患者外周血中LCAT DNA高甲基化与AS的发生发展和血脂变化相关。

关键词: 关键词:卵磷脂胆固醇酰基转移酶, DNA甲基化, 动脉粥样硬化, 血脂

Abstract: Objective To analysize the change of LCAT DNA methylation level and the relationship with blood lipids in Hui patients. Methods The 35 AS patients and 42 controls from Hui ethnic were confirmed by carotid artery Doppler ultrasound, LCAT mRNA expression were determined by real-time reverse transcription–polymerase chain reaction (qRT-PCR). The methylation status of LCAT gene promoter region was studied using the nest tounchdown methylation specific polymerase chain reaction (nt-MSP). The serum lipids were measured by ADVIA 2400 Automatic Biochemistry Analyzer. Results The TC,TG and LDL were higher than the normal group, HDL were lower than the normal group. Besides, compared with the normal group, the LCAT mRNA level of AS patients decreased by 61.2%(P<0.01), and the LCAT DNA methylation level increased 1.3 times(P<0.05). Conclusion The LCAT DNA hypermethylation is related to the occurrence and development of AS and the changes of serum lipids in Hui patients.

Key words: Key Words: lecithin cholesterol acyltransferase, DNA methylation, atherosclerosis, blood lipids

中图分类号: