基础医学与临床 ›› 2015, Vol. 35 ›› Issue (3): 303-307.

• 研究论文 • 上一篇    下一篇

人参皂苷Rg3对人肝癌细胞增殖、迁移、黏附和凋亡的影响及其作用机制

尹天翔,王燕燕   

  1. 三峡大学第一临床医学院
  • 收稿日期:2014-08-25 修回日期:2014-10-23 出版日期:2015-03-05 发布日期:2015-03-03
  • 通讯作者: 王燕燕 E-mail:wangyy1001@163.com
  • 基金资助:
    《豆腐柴中抗肿瘤二萜活性成分及抑制肿瘤浸润转移的作用机制研究》;《虎尾轮根抗类风湿性关节炎的活性成分及作用机制研究》

Effect of Ginsenoside Rg3 on the proliferation, adhesion, migration and apotosis of human hepatoma cell lines and the mechanism

  • Received:2014-08-25 Revised:2014-10-23 Online:2015-03-05 Published:2015-03-03

摘要: 目的 研究人参皂苷Rg3对不同肝癌细胞增殖、迁移、黏附和调亡的影响及其作用机制。方法 以人肝癌细胞系HepG2、QGY细胞以及人成纤维细胞系HEL细胞为研究对象,运用MTT、细胞黏附、Transwell以及流式细胞仪观察Rg3对肝癌细胞的增殖、黏附、侵袭和转移以及细胞凋亡的影响;结合Western blot检测Rg3作用于HepG2和QGY细胞后CD44、VEGF蛋白的表达。结果 Rg3能特异性抑制HepG2和QGY肝癌细胞增殖、黏附、侵袭转移并显著诱导细胞凋亡(P<0.05);HepG2和QGY肝癌细胞暴露于Rg3后细胞中CD44和VEGF蛋白表达显著下调(P<0.05)。结论 Rg3能抑制人肝癌细胞增殖、黏附、侵袭转移并诱导细胞凋亡,其功能与CD44和VEGF蛋白表达密切相关。

关键词: 关键词:人参皂苷Rg3, 肝癌细胞, 细胞增殖, 细胞凋亡, VEGF

Abstract: Object To investigate the effect of Ginsenoside Rg3 on proliferation, adhesion, migration and apoptosis of human hepatoma cell lines and reveal its mechanism. Methods The human hepatocellular carcinoma cell line HepG2, QGY and non tumor cell line HEL cells were used. By using the MTT test, cell adhesion assay, Transwell and flow cytometry, the effect of Rg3 on hepatocellular carcinoma cell proliferation, adhesion, migration and apoptosis were observed. The expression of CD44 and vascular endothelial growth factor(VEGF) protein were detected by Western blot after HepG2 and QGY cells exposed to Rg3. Results Rg3 could specifically inhibit the cell proliferation, adhesion capacity, migration of HepG2 and QGY cells and significantly induced the apoptosis (P<0.05). The expression of CD44 and VEGF protein were significantly down-regulated after Rg3 exposed to HepG2 and QGY hepatocarcinoma cells (P?0.05). Conclusion Rg3 inhibits cell proliferation, adhesion, migration of human hepatoma cell lines and significantly induce the cell apoptosis, and these effects are closely related with the expression inhibition of CD44 and VEGF protein.

Key words: Key Word: ginsenoside Rg3, human hepatoma cell line, cell proliferation, cell apoptosis, vascular endothelial growth factor.