基础医学与临床 ›› 2015, Vol. 35 ›› Issue (11): 1535-1539.

• 研究论文 • 上一篇    下一篇

肌肽减轻H2O2诱导的N2a细胞损伤

魏建峰   

  1. 徐州医学院
  • 收稿日期:2015-06-11 修回日期:2015-09-22 出版日期:2015-11-05 发布日期:2015-11-03
  • 通讯作者: 魏建峰 E-mail:wjf@xzmc.edu.cn

Carnosine Reduces H2O2-induced cellular Injury in N2a Cells

  • Received:2015-06-11 Revised:2015-09-22 Online:2015-11-05 Published:2015-11-03

摘要: 目的 探究肌肽对H2O2诱导的N2a细胞损伤的保护作用及其机制。方法 将N2a细胞分为对照组;损伤组(给予H2O2);肌肽保护组(预先加入肌肽)。用相差显微镜观察了细胞形态,用免疫荧光染色和免疫印迹实验检测细胞AKT1和FAS-L的蛋白表达。结果 肌肽显著增加了N2a细胞增殖能力(P<0.05);与对照组比较,H2O2损伤组细胞形态发生明显改变,细胞皱缩,胞膜变厚并出现发泡现象;而肌肽保护组细胞形态较损伤组明显改善;免疫荧光染色和免疫印迹实验显示,损伤组AKT1的表达显著低于对照组,FAS-L的表达显著高于对照组。 而肌肽保护组AKT1的表达显著高于对照组,FAS-L的表达显著低于对照组(P<0.05)。结论 肌肽能够减轻N2a细胞对H2O2诱导的细胞损伤。

关键词: N2a细胞, 肌肽, 氧化应激损伤, Akt1, Fas-L

Abstract: Objective To investigate the protective effect of carnosine in oxidative stress induced by H2O2 in N2a cells. Methods The N2a cells were divided into three groups: normal control; H2O2 group; carnosine group: carnosine was used in advance. Cell morphology was observed by phase-contrast microscope; immunohistochemistry and Western blot were applied to detect expression of AKT1 and FAS-L. Results Carnosine significantly increased cell proliferation. Compared with control group,cell morphology changed significantly, cell shrinkage, membrane thickened, foaming phenomenon in H2O2 injury group; In the carnosine protection group, carnosine significantly inhibited the H2O2-induced cellular injury, cell morphology obviously better than H2O2 group. Compared with the control group, the expression of the AKT1 down-regulated and Fas-L up-regulated in H2O2 group, but in the carnosine protection group, the expression of the AKT1 up-regulated and Fas-L down-regulated than H2O2 group (P<0.05). Conclusions Carnosine obviously reduced H2O2-induced cellular injury in N2a cells.

Key words: N2a cell, carnosine, oxidative stress, Akt1, Fas-L