基础医学与临床 ›› 2014, Vol. 34 ›› Issue (9): 1221-1225.

• 研究论文 • 上一篇    下一篇

MIBG对人肝癌HepG2细胞增殖的影响

刘逢秋1,管小琴2,王娅兰1,苏晏1   

  1. 1. 重庆医科大学
    2. 重庆医科大学基础医学院
  • 收稿日期:2014-02-20 修回日期:2014-05-26 出版日期:2014-09-05 发布日期:2014-09-02
  • 通讯作者: 管小琴 E-mail:guanxiaoqin2003@yahoo.com.cn
  • 基金资助:
    高等学校博士学科点专项科研基金联合资助项目;重庆市教委科学技术研究项目

Effects of MIBG on proliferation in hepatoma carcinoma HepG2 cells

  • Received:2014-02-20 Revised:2014-05-26 Online:2014-09-05 Published:2014-09-02

摘要: 目的 探究间碘苄胍(MIBG)对肝癌细胞HepG2增殖的影响及其可能机制。方法 细胞免疫荧光法检测HepG2细胞中精氨酸特异性腺苷二磷酸核糖基化转移酶1(ART1)的表达。MTT比色实验检测肝癌细胞HepG2的生存率。流式细胞计数法检测细胞周期。Westren blot检测ART1、RhoA、c-myc和cyclinA1的表达。结果 ART1在HepG2细胞有表达。MIBG对HepG2细胞增殖抑制呈剂量依赖性(P<0.05),主要是将细胞阻滞在S期(P<0.05)。MIBG能降低ART1、RhoA、c-myc和cyclinA1的表达水平 (P<0.05)。结论 MIBG通过影响RhoA信号通路,下调c-myc蛋白和cyclinA1蛋白的表达,将细胞阻滞在S期,而抑制HepG2细胞的增殖。

关键词: 肝癌, 细胞增殖, 单腺苷二磷酸核糖基化, 间碘苄胍

Abstract: Objective To study the effects of MIBG(meta-iodobenzylguanidine) on proliferation in hepatoma carcinoma HepG2 cells and its possible mechanism. Methods The expression of ART1 in HepG2 cells was detected by cellular immunofluorescence method. The effect of different concentration of MIBG on cell survival rate and cell cycle phases in HepG2 cells were respectively measured by MTT assay and flow cytometry. The expression of ART1, RhoA , c-myc and cyclinA1 were detected by Western blot. Results It showed that the ART1 existed in the HepG2 cells. The MIBG could inhibit the growth of the HepG2 cells arrested in S phase, which could be affected by different concentrations (P<0.05). The expression of the ART1, RhoA, c-myc and cyclinA1 decreased after using the MIBG (P<0.05). Conclusion MIBG, which down-regulated RhoA pathway and the downstream factors c-myc and cyclinA1, could inhibit the proliferation of HepG2 cells and arrest the cell cycles in S phase.

Key words: Hepatoma carcinoma, cell proliferation, mono-ADP-ribosylation, MIBG