基础医学与临床 ›› 2014, Vol. 34 ›› Issue (11): 1472-1476.

• 研究论文 • 上一篇    下一篇

系统性红斑狼疮患者血清乳铁蛋白的分析

马友楠1,高瑞通2,吴凤岐3,高凤英4,张伟1   

  1. 1. 中国医学科学院基础医学研究所
    2. 中国医学科学院北京协和医学院北京协和医院
    3. 首都儿科研究所
    4. 三河市儿童医院
  • 收稿日期:2014-04-24 修回日期:2014-07-17 出版日期:2014-11-05 发布日期:2014-11-03
  • 通讯作者: 张伟 E-mail:wzhang@pumc.edu.cn
  • 基金资助:
    国家自然科学基金

The analysis of serum lactoferrin levels in patients with systemic lupus erythematosus

  • Received:2014-04-24 Revised:2014-07-17 Online:2014-11-05 Published:2014-11-03
  • Supported by:
    the Chinese National Natural Science Foundation

摘要: 目的 比较健康人和系统性红斑狼疮(SLE)患者血清中乳铁蛋白(LF)的水平,分析SLE患者LF降低的影响因素。方法 采用ELISA检测健康儿童(n=38)、健康成人(n=46)、SLE儿童患者(n=38)、SLE成人患者(n=46)、1~80岁不同年龄段健康人(n=80)、IgA肾病患者(n=29)及其他肾病患者(n=19)血清中LF的含量。BCA法检测血清中总蛋白的含量。非配对t检验分析不同组间LF水平的差异,Spearman’s相关性分析研究血清中LF与总蛋白含量间的关系。结果 SLE儿童及成人患者血清中LF水平均显著低于年龄和性别匹配的健康儿童及成人(P <0.001),也低于IgA肾病和其他肾病患者(P <0.001)。51~80岁健康人血清LF含量低于1~50岁健康人。结论 SLE患者血清中LF水平显著降低,此降低不是由于血清中总蛋白含量变化引起的。

关键词: 关键词:系统性红斑狼疮, 乳铁蛋白, 生物标志物

Abstract: Objective To compare serum levels of lactoferrin (LF) in healthy subjects and patients with systemic lupus erythematosus (SLE). To analyze the cause for reduced LF levels in SLE patients. Methods Serum concentrations of lactoferrin in healthy children (n = 38), healthy adults (n = 46), pediatric SLE patients (n = 38), adult SLE patients (n = 46), healthy people with ages from 1 to 80 (n = 80), IgA nephropathy patients (n = 29) and other kidney patients (n = 19) were measured by ELISA. Total protein concentrations in serum were measured by BCA. An unpaired t test was performed to analyze the differences between two groups and Spearman's correlation analysis was performed to analyze the relationship between the levels of LF and total protein. Results Serum concentrations of LF in both pediatric and adult patients were lower than that in healthy control subjects, patients with IgA nephropathy and other kidney patients (P<0.001). The levels of LF were reduced in people aged over 50. Conclusion LF levels were significantly reduced in SLE patients, which was not caused by the changes of total protein in serum.

Key words: Key words: Systemic Lupus Erythematosus, Lactoferrin, Biomarker

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