基础医学与临床 ›› 2014, Vol. 34 ›› Issue (11): 1468-1471.

• 研究论文 • 上一篇    下一篇

类叶升麻苷通过诱导血红素加氧酶-1的表达抑制MPP+诱导的PC12细胞损伤

王洪权1,王玉敏2,崔其福2,赵伟丽2,成龙3   

  1. 1. 赤峰学院(附属医院)
    2. 赤峰学院附属医院
    3. 中国医学科学院 药用植物研究所
  • 收稿日期:2014-04-10 修回日期:2014-06-20 出版日期:2014-11-05 发布日期:2014-11-03
  • 通讯作者: 王洪权 E-mail:whongquan@aliyun.com
  • 基金资助:
    Nrf2/ARE/HO-1通路在松属素抑制Aβ诱导的氧化损伤中的作用机理研究;内蒙古教育厅青年科技英才支持计划;松属素在帕金森病MPP+模型中的神经保护作用机制研究

Acteoside attenuate MPP+-induced PC12 cell injury via induction of heme oxygenase-1 expression

  • Received:2014-04-10 Revised:2014-06-20 Online:2014-11-05 Published:2014-11-03
  • Contact: Hongquan Wang E-mail:whongquan@aliyun.com

摘要: 目的 探讨类叶升麻苷(AS) 是否在PC12细胞中诱导血红素加氧酶-1(HO-1)的表达进而抑制1-甲基-4-苯基吡啶(MPP+)诱导的神经毒性损伤。方法 类叶升麻苷(1、20和30 μmol/L)处理 PC12细胞12 h,利用反转录PCR(RT-PCR)检测HO-1 mRNA表达、Western blot方法和免疫荧光方法检测HO-1蛋白的表达。1mmol/L MPP+单独或与AS处理细胞,或HO-1抑制剂锌原卟啉(ZnPP)预处理细胞1 h,MTT法检测细胞活力。结果 与正常对照组相比, AS可在PC12细胞中诱导HO-1 mRNA和蛋白的表达,AS诱导的HO-1表达主要集中在细胞浆内。HO-1抑制剂ZnPP减弱AS对MPP+诱导损伤的抑制作用。结论 AS可在PC12细胞中诱导HO-1的表达,可能参与AS对MPP+诱导损伤的抑制作用。

关键词: 类叶升麻苷, 血红素加氧酶-1, 1-甲基-4-苯基吡啶, 神经保护

Abstract: Objective To investigate whether acteoside (AS) protect PC12 cells against MPP+ -induced neurotoxicity through induction of HO-1 expression. Methods PC12 cells were stimulated with AS(1、20 and 30 μmol/L) for 12 h, RT-PCR was used to analyze HO-1 mRNA expression, and western blot and confocal microscopic analysis was applied to determine the expression of HO-1 protein. Cells were pre-incubated with vehicle or AS for 2h, followed by incubation with 1 mmol/L MPP+ for another 24 h,or cells were pretreated with Zinc Protoporphyrin (ZnPP) for 1 h in the absence or presence of AS and were exposed to MPP+ for 24 h,cell viability was measured by MTT assay. Results AS significantly reduced MPP+-induced the loss of cell viability. AS induced the expression of HO-1 in PC12 cells at mRNA and protein level. Acteoside-induced HO-1 which was predominantly localized to the cytoplasm. The HO-1 inhibitor ZnPP markedly abolished the neuroprotective effect of AS against MPP+-induced neurotoxicity. Conclusions Acteoside induce HO-1 expression, thereby attenuating MPP+-induced PC12 cell injury.

Key words: Acteoside, Heme oxygenase-1, MPP+, Neuroprotection

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