PTTG1通过上皮-间质转化促进非小细胞肺癌的侵袭和转移

基础医学与临床 ›› 2014, Vol. 34 ›› Issue (10): 1376-1380.

PTTG1通过上皮-间质转化促进非小细胞肺癌的侵袭和转移

李洪利¹,尹崇高²

1. 山东省潍坊医学院医学研究实验中心
2. 山东省潍坊医学院护理学院

收稿日期:2014-02-27 修回日期:2014-04-22 出版日期:2014-10-05 发布日期:2014-09-25
通讯作者: 尹崇高 E-mail:ycg0711@sina.com
基金资助:
山东省中青年科学家科研基金（博士基金）;山东省高等学校科技计划

PTTG1 Induces Migration And Invasion Of Non-small Cell Lung Cancer Through Epithelial-mesenchymal Transition

Received:2014-02-27 Revised:2014-04-22 Online:2014-10-05 Published:2014-09-25

摘要/Abstract

摘要： 目的研究PTTG1在非小细胞肺癌侵袭转移中的作用。方法应用免疫组织化学染色法检测80例NSCLC组织中PTTG1、E-cadherin和Vimentin的表达，并分析其相关性。细胞分组：1）A549/con，转染空载的A549，2）A549/PTTG1，转染目的基因的A549，3）Scr/H1299，转染空载的H1299，4）SiPTTG1/H1299，敲除PTTG1的H1299；用Western blot检测细胞中PTTG1、E-cadherin、Vimentin蛋白的表达情况。结果 NSCLC组织中PTTG1蛋白的阳性表达率为85%与E-cadherin的表达呈负相关（P＜0.05）而与Vimentin的表达呈正相关(P＜0.05)。Western blot结果显示在SiPTTG1/H1299细胞中PTTG1蛋白表达水平明显降低，Vimentin蛋白表达水平降低而E-cadherin蛋白表达水平升高。同时在A549/PTTG1细胞中Vimentin蛋白表达水平升高而E-cadherin蛋白表达水平降低。结论 PTTG1能促进NSCLC EMT的发生，从而促进NSCLC的侵袭和转移。

关键词: 垂体肿瘤转化基因1, 上皮-间质转化, 肺肿瘤, 侵袭

Abstract: Objective To investigate the role of PTTG1 in the migration and invasion of Non-small cell lung cancer. Methods To detect the expression of PTTG1, E-cadherin and Vimentin in 80 cases of NSCLC using immunohistochemistry. Cells were divided by four groups : ①A549/con, cells were transfected by empty vector②A549/PTTG1, cells were transfected by purpose gene ③Scr/H1299, cells were transfected by empty vector ④SiPTTG1/H1299, cells were knocked down purpose gene. Western blot was used to analyze the expression of PTTG1, E-cadherin and Vimentin. Results The protein expression of PTTG1 in NSCLC was negatively correlated with E-cadherin and positively correlated with Vimentin (P<0.05). H1299 cells expressed higher PTTG1, and A549 cells expressed lower PTTG1. The expressions of PTTG1 and Vimentin were lower in SiPTTG1/H1299 cells than those in Scr/H1299 and H1299 cells after trancfected 24 hours, but the E-cadherin expression was higher. At the same time, the protein expression of Vimentin was up-regulated and the expression of E-cadherin was down-regulated in A549/PTTG1 cells. Conclusions PTTG1 can promote migration and invasion of NSCLC through EMT.

Key words: PTTG1, EMT, Lung tumor, migration

中图分类号:

李洪利尹崇高. PTTG1通过上皮-间质转化促进非小细胞肺癌的侵袭和转移[J]. 基础医学与临床, 2014, 34(10): 1376-1380.

[1]	孙光炎丁明炎姚和迁曾凡杨. HMG-CoA2裂解酶在鼻咽癌组织中表达及其对鼻咽癌细胞增殖、侵袭能力的影响[J]. 基础医学与临床, 2019, 39(9): 1294-1299.
[2]	武永强杨帆. 干扰Arf6抑制人前列腺癌细胞系DU145增殖、迁移及侵袭[J]. 基础医学与临床, 2019, 39(9): 1310-1315.
[3]	张杨杜雯雯刘婷曾园园黄建安. miR-195-5p表达上调抑制肺癌细胞系A549的迁移和侵袭[J]. , 2019, 39(12): 1694-1698.
[4]	施能杨欣谕. miR-195抑制舌鳞癌细胞系CAL27增殖、迁移和侵袭[J]. , 2019, 39(12): 1689-1693.
[5]	陈素华马天江张智慧张磊史磊. 淫羊藿苷通过下调转移相关蛋白2抑制MGC803细胞的增殖、迁移和侵袭[J]. , 2019, 39(11): 1587-1591.
[6]	王盛华付民赵高峰. 七氟烷抑制人乳腺癌细胞系MCF-7增殖、迁移和侵袭[J]. , 2019, 39(11): 1597-1602.
[7]	方复毛华辉卢国春郭渊童向美. IL-8在胃癌组织上皮间质转化过程中表达增强[J]. 基础医学与临床, 2019, 39(10): 1444-1448.
[8]	刘桂元张永慧庞毅. WNT信号抑制因子DKK3基因对结直肠癌细胞系HCT116迁移与侵袭的影响[J]. 基础医学与临床, 2019, 39(10): 1437-1443.
[9]	涂其武沈晓黎. miR-34a通过MMP2抑制脑胶质瘤细胞侵袭和转移[J]. 基础医学与临床, 2018, 38(8): 1115-1120.
[10]	张达容王维莲吴立翔柳满然. Twist促进人三阴性乳腺癌细胞迁移和侵袭[J]. 基础医学与临床, 2018, 38(6): 771-775.
[11]	张洋张静季琳李文媛王莹孙平于伟光. TRIM11靶向调控miR-24-3p促进乳腺癌MCF7细胞系增殖和侵袭[J]. 基础医学与临床, 2018, 38(4): 451-457.
[12]	邹子归周晋星马天时张智弘. 长链非编码RNA调控乳腺癌侵袭与转移的研究进展[J]. 基础医学与临床, 2018, 38(4): 573-577.
[13]	蔡志宏曹怡静刘姣胡皓彬谢鑫许雄峰凌晖. 转染胃动蛋白2基因抑制人胃癌MKN28细胞系增殖、迁移和侵袭[J]. 基础医学与临床, 2018, 38(3): 370-374.
[14]	邓秀娟韩铭刘顺爱成军梁跃东. 乙型肝炎病毒反式激活因子XTP4促进肝癌细胞系迁移和侵袭[J]. 基础医学与临床, 2018, 38(12): 1718-1723.
[15]	陈宛丽关红梅王舒. 硼替佐米促进霍奇金淋巴瘤细胞系L428的凋亡[J]. 基础医学与临床, 2018, 38(10): 1438-1442.