基础医学与临床 ›› 2013, Vol. 33 ›› Issue (9): 1186-1190.

• 研究论文 • 上一篇    下一篇

大鼠胎盘间充质干细胞降低大鼠缺血低氧性脑损伤

丁红芳1,丁慧芳2,高欣义1,伊心浩1,鞠秀丽3   

  1. 1. 胜利油田中心医院儿科
    2. 胜利油田中心医院
    3. 山东大学齐鲁医院
  • 收稿日期:2012-08-13 修回日期:2012-11-28 出版日期:2013-09-05 发布日期:2013-08-28
  • 通讯作者: 丁慧芳 E-mail:cupdocmeng@126.com

Transplantation of placenta-derived mesenchymal stem cells reduces hypoxic-ischemic brain injury in rats

  • Received:2012-08-13 Revised:2012-11-28 Online:2013-09-05 Published:2013-08-28

摘要: 目的 探讨胎盘间充质干细胞(PDMSCs)治疗新生大鼠低氧缺血性脑病(HIBD)的效果及其作用机制。方法 HIBD模型乳鼠随机分为对照组、HIBD组、HIBD+PDMSCs治疗组和HIBD+成纤维细胞治疗组。细胞治疗后14d评估各组生长发育及神经功能,用RT-PCR法和ELISA法检测海马和外周血中TNF-a、IL-17、IFN-γ和IL-10的变化。结果 HIBD组损伤侧大脑明显萎缩、神经行为严重障碍,发育较对照组明显落后(P<0.05),PDMSCs治疗后脑萎缩、神经行为障碍均改善,且幼鼠的体质量显著升高(P<0.05)。细胞治疗后14d,HIBD组海马和外周血中TNF-a、IL-17和IFN-γ较对照组均升高,而IL-10水平降低(P<0.05);PDMSCs治疗组上述改变显著(P<0.05)。结论 PDMSCs治疗能够改善新生HIBD大鼠的神经功能和发育,其作用机制可能是通过减轻机体的炎性反应。

关键词: 间质干细胞, 胎盘, 低氧缺血, 脑, 炎性

Abstract: Objective In the present study, we investigate the neuroprotective effect of placenta-derived mesenchymal stem cells (PDMSCs) transplantation in rat's hypoxic-ischemic brain damage(HIBD) model and its mechanism. Methods The rats were randomly divided into four groups: sham-operated group; only HIBD group; HIBD+PDMSCs-treated group and HIBD+Fibroblasts-treated group. After 2 weeks of treatment, we measured inflammatory cytokines (TNF-a, IFN-γ, IL-10 and IL-17) expression in the brain and serum by appropriate methods. Results Our results indicated that pro-inflammatory cytokine gene and protein expressions increased in the HIBD group, and that PDMSCs-treated group significantly (all P<0.05) lowered at these levels. Furthermore, PDMSCs treatment was able to increase IL-10 levels. We also observed that motor dysfunctions in Fibroblasts-treated group were not improved and the indicators were not statistically significant compared with PDMSCs-treated group. Conclusions In summary, PDMSCs treatment plays a critical role in the neuroprotection observed in this model and the mechanism may be partly through alleviating inflammatory reaction.

Key words: Key words: mesenchymal stem cells, placenta, hypoxic-ischemic, brain, inflammatory reaction

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