基础医学与临床 ›› 2013, Vol. 33 ›› Issue (2): 211-214.

• 研究论文 • 上一篇    下一篇

非肥胖糖代谢异常大鼠血清的代谢组学

辛衍代1,臧学章1,季虹2,荣海钦2   

  1. 1. 山东省内分泌与代谢病研究所
    2. 济南山东省内分泌与代谢病研究所
  • 收稿日期:2011-10-17 修回日期:2012-05-30 出版日期:2013-02-05 发布日期:2013-01-25
  • 通讯作者: 荣海钦 E-mail:haiqinrong@126.com
  • 基金资助:
    山东省医学科学院院级项目

Serum Metabonomic Profiling of Obesity-Resistant Rats with glucose metabolism disorders

  • Received:2011-10-17 Revised:2012-05-30 Online:2013-02-05 Published:2013-01-25

摘要: 目的 探讨非肥胖型2型糖尿病的发病机制。方法 建立肥胖抵抗大鼠模型,取静脉血,测生化指标,对血清进行代谢组学分析。结果 大鼠在高糖高脂饮食饲养16周后,与基础饲料组相比,血糖、胰岛素、低密度脂蛋白及总胆固醇显著升高,高密度脂蛋白显著下降,同时糖酵解产物丙酮酸降低,三羧酸循环的中间代谢物柠檬酸、苹果酸、延胡索酸及琥珀酸减少,尿酸增加,脂肪酸、氨基酸代谢异常。结论 高糖高脂饮食干预后,肥胖抵抗大鼠能量代谢显著变化,对探讨饮食导致的非肥胖型2型糖尿病发病机理的研究有重要意义。

关键词: 肥胖抵抗, 代谢组学, 气相色谱-质谱, 糖代谢

Abstract: Objective We investigated the major metabolic changes in low molecular weight serum metabolites of Obesity resistant ratsand control rats, understanding the pathogenesis of non-obese type 2 diabetes. Methods A obesity resistant rat model was constructed and the biochemical criteria were analyzed. The metabolites in the serum were exhaustively separated and detected by GC-MS. Multivariate metabonomics analysis was performed after peak alignment. Results Compared with control rats, the obesity resistant rats showed clear metabolism dysfunctions of glucose, lipid and amino acid, pyruvate, succinate, fumarat, citric acid, myristic acid, uric acid were decreased. And serum sugar, insuline,low-density lipoprotein and total cholesterol was significantly increased,high density lipoprotein, triglyceride decreased significantly. Conclusions After 16 weeks of dietary intervention, the energy metabolism of obesity resistant rats show significant change. The metabolite profiling study could be helpful for understanding mechanism of non-obese type 2 diabetes.

Key words: Obesity resistant, Metabonomics, GC-MS, glucose metabolism

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