基础医学与临床 ›› 2013, Vol. 33 ›› Issue (11): 1398-1403.

• 间充质干细胞与皮肤损伤修复专题 • 上一篇    下一篇

聚乙烯亚胺包被的Fe3O4磁纳米颗粒对hADSCs生物学特性的影响

王瑞1,刘晓玉1,庞希宁1,施萍2   

  1. 1. 中国医科大学
    2. 中国医科大学附属第一医院
  • 收稿日期:2013-08-11 修回日期:2013-09-17 出版日期:2013-11-05 发布日期:2013-10-28
  • 通讯作者: 庞希宁 E-mail:pangxining@126.com
  • 基金资助:
    国家重点基础研究发展规划;辽宁省科学技术计划;沈阳市科学技术计划

Effects of covered polyethylenimine Fe3O4 superparamagnetic iron oxide on human adipose mesenchymal stem cells

  • Received:2013-08-11 Revised:2013-09-17 Online:2013-11-05 Published:2013-10-28

摘要: 目的 探讨聚乙烯亚胺(PEI)包被的超顺磁性氧化铁(SPIO)对人脂肪间充质干细胞(hADSCs)生物学特性的影响。方法 从人皮下脂肪组织中分离、培养hADSCs并鉴定,选取第3代hADSCs,分别用含铁浓度为6、8、10和12mg/L的DMEM/F12培养液共孵育。通过普鲁士蓝染色、 MTT细胞增殖实验、台盼蓝染色及多向分化诱导实验来探讨PEI-SPIO标记后,hADSCs的标记能力、细胞增殖能力和分化能力。结果 1)当铁终浓度为8、10和12mg/L时,PEI-SPIO对hADSCs的标记率为99% ,当铁终浓度<8mg/L时,PEI-SPIO对hADSCs的标记率为96% 。2)当铁终浓度为6和8mg/L时, PEI-SPIO标记的hADSCs的活细胞比率为99%,而当铁终浓度为10和12mg/L时,活细胞比率为(79.03±2.25)%和(70.23±1.36)%,显著低于对照组(P<0.01)。3)当铁浓度为10和12mg/L时,在诱导hADSCs分化过程中,细胞绝大多数死亡。结论 铁浓度8mg/L为PEI-SPIO标记的最适宜浓度,既能高效的标记hADSCs,又不影响hADSCs的细胞增殖、多向分化能力等生物学特性。

关键词: 关键词:人脂肪间充质干细胞, 聚乙烯亚胺, 超顺磁性氧化铁, 生物学特性

Abstract: Objective To investigate the effects of superparamagnetic iron oxide covered by polyethylenimine(PEI) on human adipose mesenchymal stem cells (hADSCs).Methods The adipose tissue come from human subcutaneous,we extraction separation, culture and identification of hADSCs .The third passage of hADSCs were divided into four groups,and incubated in DMEM/F12 containing PEI -SPIO of 6,8,10,12mg/L.Detected by Prussian blue staining,Trypan blue staining,MTT test and inductions of multi-directional differentiation to evaluate the ability of SPIO on cell labeling efficiency,cell viability,proliferation and differentiation. Results 1.The labeling efficiency of SPIO (8,10,12mg/L) was 99%.However the labeling efficiency of SPIO(6mg/L)was 96%.2.In 6 ,8μg /ml group,the cell survival rate was 99%. But in10mg/L and 12mg/L group,the cell survival rate was(79.03±2.25)% and (70.23±1.36)%, Significantly lower than the control group(P<0.01).3.In 10,12 mg/L group,there were statistically significant differences in the viability,most of cells deceased in the procedure of multi-directional differentiation.Conclusions PEI-SPIO of 8mg/L can effectively label hADSCs,without influencing the cell viability,proliferation and the multiple differentiation ability.

Key words: Key words:Human adipose mesenchymal stem cells, Polyethylenimine, Superparamagnetic iron oxide, Biological characteristic

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