基础医学与临床 ›› 2013, Vol. 33 ›› Issue (11): 1377-1381.

• 间充质干细胞与皮肤损伤修复专题 • 上一篇    下一篇

人脂肪间充质干细胞对皮肤创伤修复的作用

刘晓玉1,王瑞1,张涛1,庞希宁1,施萍2   

  1. 1. 中国医科大学
    2. 中国医科大学附属第一医院
  • 收稿日期:2013-08-27 修回日期:2013-09-16 出版日期:2013-11-05 发布日期:2013-10-28
  • 通讯作者: 庞希宁 E-mail:pangxining@126.com
  • 基金资助:
    国家重点基础研究发展计划;辽宁省科学技术计划;沈阳市科学技术计划

Effects of Human Adipose-derived Mesenchymal Stem Cells on Cutaneous Wound Healing

Xiao-Yu LIU1,rui WANGTao ZHANG1, ping shi   

  • Received:2013-08-27 Revised:2013-09-16 Online:2013-11-05 Published:2013-10-28

摘要: 目的 探讨人脂肪间充质干细胞(hADSCs)皮内移植对小鼠皮肤创面修复的作用,为临床皮肤创伤后修复提供新的细胞治疗方法奠定基础。方法 分离培养hADSCs,取3~5代细胞通过免疫荧光法检测间充质干细胞相关的细胞表面标志物CD90、CD105、CD34和CD45的表达,并分别置于骨、软骨和脂肪诱导分化培养基中进行诱导分化,检测其多向分化潜能。在实验小鼠背部制备一个面积为1cm×1cm的皮肤创面,将磁性纳米颗粒标记的hADSCs以皮内注射方式移植到小鼠创面四周。分别于伤后0、7和14d观察创面愈合情况, 并与对照组进行比较分析。结果 人脂肪间充质干细胞表达CD90和CD105,不表达CD34和CD45,具有骨,软骨,脂肪诱导分化潜能;实验小鼠皮肤创伤后3d开始,可见各组创面逐渐缩小,伤后21d创面愈合。移植组于创伤后第7d创面愈合率为(74.6%±4.1%),14d为(96.5%±1.47%),均显著高于对照组7d的(26.7%±1.9%),14d的(47.3%±2.3%),(P<0.05)。结论 hADSCs移植能够提高小鼠皮肤创面愈合的速度。

关键词: 脂肪, 间充质干细胞, 创伤愈合

Abstract: Objective To explore the effects of adipose derived mesenchymal stem cells(hADSCs)on cutaneous wound healing and provide a new method for clinical skin repair in the future. Methods The hADSCs were isolated by mechanical separation and subsequent enzymatic digestion. The expression profiles of CD90,CD105,CD34andCD45 were examined by immunofluorescence. The hADSCs from passage 3-5 were used for bone, osteogenic, adipogenic differentiation assays by culturing in appropriate induction medium.The mice were anesthetized and a 12-cm full-thickness excisional skin wound was made on the back of each mouse. After labeling with superparamagnetic iron oxide nanoparticles (SPIONs ), hADSCs were injected intradermally around the wound at four different injection sites. After o,7and 14d , the area of the wounds were measured and performed to evaluate the quality of wound healing compare with control. Results The morphological feature of hADSCs displayed fibroblast-like phenotype.The cells were positive for stem cells markers,including CD90 and CD105,and negative for CD34 and CD45,as shown by immunofluorescence. Osteogenic,chondrogenic, adipogenic differentiations were revealed in hADSCs when cultured in specific medium.The first 3 days of the experimental mice in each group after skin wound, wound gradually reduced, most of the wounds healed after 21d. Compared with the control group (26.7%±1.9)at 7d and(47.3%±2.3)at 14d,, the most obvious effect at 7d (74.6%±4.1)and 14d(96.5%±1.47)after transplantation(P<0.05). Conclusion hADMSCs transplantation can improve wound healing.

Key words: adipose, mesenchymal stem cells, wound healing

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