基础医学与临床 ›› 2013, Vol. 33 ›› Issue (1): 98-101.

• 研究论文 • 上一篇    下一篇

噻托溴铵抑制瘦素促大鼠气道平滑肌细胞的增殖

吴瑕1,朱述阳1,郑玉龙2,刘文静1,陈云峰1,倪文静1,赵玲1   

  1. 1. 徐州医学院附属医院
    2. 淮安市第二人民医院
  • 收稿日期:2012-02-10 修回日期:2012-05-02 出版日期:2013-01-05 发布日期:2012-12-25
  • 通讯作者: 朱述阳 E-mail:jiechenz356@sohu.com

Tiotropium inhibition of the proliferation of airway smooth muscle cells stimulated by leptin in rats

  • Received:2012-02-10 Revised:2012-05-02 Online:2013-01-05 Published:2012-12-25

摘要: 目的 探讨噻托溴铵对瘦素促大鼠气道平滑肌细胞(ASMCs)增殖的作用。方法 体外培养大鼠ASMCs,瘦素(100 μg /L)干预后,加入不同浓度噻托溴铵(μg/L)(15、30、60和120),CCK-8法测定ASMCs增殖,荧光实时定量RT-PCR及免疫印迹(Westernblot)分别检测瘦素受体mRNA及蛋白含量水平表达。结果 与对照组比较,瘦素可明显促进大鼠ASMCs增殖(P<0.05),加入噻托溴铵则抑制瘦素的促增殖作用(P<0.05),并与浓度相关(P<0.01)。瘦素作用后,其受体mRNA表达增加,蛋白含量由对照组的0.327±0.011 升至0.541±0.016 (P<0.05);噻托溴铵干预后,瘦素受体mRNA表达减少,蛋白含量分别回降至(0.514±0.017、0.468±0.025、0.331±0.023、0.313±0.017)(P<0.05);并与浓度相关(P<0.01)。结论 噻托溴铵可抑制瘦素的促ASMCs增殖作用,其机制可能与下调大鼠ASMCs表面的瘦素受体表达有关。

关键词: [关键词]:噻托溴铵, 瘦素, 瘦素受体, 气道平滑肌, 增殖

Abstract: Objective To explore the effects of tiotropium on the proliferation of rats’ airway smooth muscle cells by leptin stimulating. Methods ASMCs were derived from rat airway tissue and cultured in vitro. Then ASMCs were treated with leptin (the final concentration 100 μg/L) and tiotropium at different concerntrations (μg/L) (15、30、60 and 120) and cell proliferation was measured by CCK-8 assay. Realtime RT-PCR and Western blot were used to verify the expressions of leptin receptors on ASMCs. Results Leptin could promote the proliferation of ASMCs compared with control group (P<0.05). After treated with tiotropium, leptin’s promotion of the proliferation of ASMCs was inhibited in a dose-dependent manner (P<0.01). After treated with leptin, the expressions of leptin receptor mRNA were increased and the proteins were rose from 0.327±0.011 to 0.541±0.016 (P<0.05); then treated with different concerntrations of tiotropium, the expressions of leptin receptor mRNA were decreased and the proteins were reduced respectively to 0.514±0.017、0.468±0.025、0.331±0.023 and 0.313±0.017(P<0.05). The expressions of leptin receptors were positively related to tiotropium concentrations (P<0.01). Conclusion Tiotropium can inhibit the proliferation of rats’ airway smooth muscle cells stimulated by leptin, and its mechanism may be correlated to the decreased the expression of leptin receptors on rats’ ASMCs.

Key words: [Key word]: tiotropium, leptin, leptin receptor, airway smooth muscle cells, proliferation

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