基础医学与临床 ›› 2013, Vol. 33 ›› Issue (1): 93-97.

• 研究论文 • 上一篇    下一篇

LATS2基因在上皮性卵巢癌中的表达和临床意义

雷静1,万霖1,李惠新2   

  1. 1. 兰州大学第二临床医学院
    2. 兰州大学第二医院
  • 收稿日期:2011-11-16 修回日期:2012-05-03 出版日期:2013-01-05 发布日期:2012-12-25
  • 通讯作者: 李惠新 E-mail:lihx527@163.com

The expression and clinical significance of LATS2 in epithelial ovarian cancer

  • Received:2011-11-16 Revised:2012-05-03 Online:2013-01-05 Published:2012-12-25

摘要: 目的 检测人上皮性卵巢癌组织中大型肿瘤抑制因子2(LATS2)的表达与其发生、发展及临床病理参数之间的相关性。方法 用RT-PCR、SYBR Green实时定量PCR及免疫组化检测LATS2mRNA和蛋白的表达。结果 LATS2mRNA在上皮性卵巢癌组织中的表达量低于良性组和正常组(P<0.01);LATS2的表达水平与组织分化程度(P<0.001)、FIGO分期(P<0.01)、有淋巴结转移(P<0.01)有关。LATS2蛋白主要定位于胞质;上皮性卵巢癌LATS2的阳性表达率均低于良性组和正常组(P<0.01);LATS2蛋白在临床晚期、组织分化低及有淋巴转移组中的阳性表达率低于临床期别早、组织分化高及无淋巴结转移组(P<0.01)。结论LATS2基因的表达水平可能与上皮性卵巢癌的发生、发展相关,对其早期诊断及预测病情进展具有一定的意义。

Abstract: Objective To detect the expression of the large tumor suppressor homolog 2(LATS2) in human epithelial ovarian cancer tissue and to analysis the correlation with the tumor progression. Methods RT-PCR、SYBR Green quantitative real-time PCR and Immunohistochemistry were employed to detect the expression the of LATS2 mRNA and protein. Results The expression of LATS2 mRNA in epithelial ovarian cancer was significant lower than that in benign,and normal group(P<0.05),and there are obvious connection between stage and metastasis of lymph node (P<0.05).The LATS2 was mainly located in cytolymph .The expression of LATS2 protein in epithelial ovarian cancer group was significant lower than that in the other groups(P<0.05).There are same results between the different stage and metastasis of lymph node. Conclusion LATS2 might play a role in the tumorigenesis of epithelial Ovarian cancer. It maybe has certain progression for early diagnosis and forecast of ovarian cancer.