基础医学与临床 ›› 2012, Vol. 32 ›› Issue (10): 1171-1174.

• 研究论文 • 上一篇    下一篇

MMP-1、MMP-2在盆底器官脱垂疾病患者阴道组织中的表达及意义

王凤玫1,王长楠2,宋岩峰3   

  1. 1. 南京军区福州总医院妇产科
    2. 第二军医大学
    3. 南京军区福州总医院
  • 收稿日期:2011-05-25 修回日期:2012-02-05 出版日期:2012-10-05 发布日期:2012-09-28
  • 通讯作者: 王凤玫 E-mail:carnation1002@yahoo.com.cn
  • 基金资助:
    福建省青年人才项目

Matrix metalloproteinases-1,-2 expression in vaginal tissues from women with pelvic organ prolapse

  • Received:2011-05-25 Revised:2012-02-05 Online:2012-10-05 Published:2012-09-28

摘要: 目的 研究基质金属蛋白酶MMP-1、MMP-2在盆底器官脱垂患者阴道组织中的表达情况,从而探讨基质金属蛋白酶与女性盆底障碍性疾病( PFD) 的关系。方法 选择我院妇产科收治的盆底器官脱垂患者20例为研究组,20例非盆底器官功能障碍患者作为对照。RT-PCR方法检测不同组MMP-1mRNA 的表达情况,明胶底物酶方法测定阴道前壁组织中MMP-1、MMP-2的表达。结果 脱垂患者阴道组织中MMP-1的表达明显增加,但是两组患者阴道壁组织中MMP-2的表达没有明显差别,而MMP-2前体在脱垂患者阴道壁组织中表达明显增加。结论 MMP-1使盆底组织胶原代谢分解增加,增加的MMP-1表达与盆底器官脱垂有关。

关键词: 金属蛋白酶, 盆底功能障碍

Abstract: Objective Compare the expression of Matrix metalloproteinase (MMP)-1 and -2 in the vaginal tissue from 20 women with pelvic floor dysfunction and the controls in order to investigate the relation of Matrix metalloproteinase and female pelvic floor dysfunction. Methods A total of 40 women referred for pelvic reconstruction surgery for pelvic floor dysfunction or benign gynaecological disease were included in this study from January 2009 to May 2010. During surgery, vaginal tissue biopsies were obtained from patients with POP (n=20) and women without evidence of pelvic floor weakening (n=20). Level of MMP-1, -2 and pro-MMP-2 in the vaginal tissue of women were measured. Statistical evaluations were made by Student t-test or x2 test. Results A significant increase in MMP-1 expression was seen in vaginal tissue from women with POP (p<0.05). In contrast, there was no difference in the expression of MMP-2 between women with POP and those without (p>0.05). Interesting, a significant increase in pro-MMP-2 expression was seen in vaginal tissue from women with POP (p<0.05). Conclusions MMP-1 increases the collagen degradation. Increased MMP-1 expression in the vaginal tissue is associated with urogenital prolapse.

Key words: Matrix metalloproteinases, pelvic organ prolapse