基础医学与临床 ›› 2012, Vol. 32 ›› Issue (1): 87-88.

• 研究短文 • 上一篇    下一篇

乳腺癌中MTDH的表达及意义

尹崇高1,高乃尧2,2,李洪利2,2,李文通3   

  1. 1. 山东省潍坊医学院护理学院
    2.
    3. 潍坊医学院
  • 收稿日期:2011-01-17 修回日期:2011-05-25 出版日期:2012-01-05 发布日期:2011-12-28
  • 通讯作者: 李文通 E-mail:liwentong11@163.com
  • 基金资助:
    山东省高等学校科技计划项目

Expression and significance of MTDH in the breast cancer

  • Received:2011-01-17 Revised:2011-05-25 Online:2012-01-05 Published:2011-12-28

摘要: 目的:通过检测乳腺癌组织中metadherin(MTDH)的表达,探讨MTDH在乳腺癌发生发展及转移中的作用。方法:采用免疫印迹及RT-PCR检测乳腺癌及癌旁乳腺组织(>5cm)中MTDH蛋白及mRNA的表达,并分析其蛋白表达与临床病理参数之间的关系。结果:乳腺癌组织中MTDH 蛋白的阳性表达率高于癌旁正常组织(P<0.05),且癌组织中MTDH蛋白阳性表达率与淋巴结转移有关(P<0.05),与TNM分期、分化程度无关(P>0.05);乳腺癌组织中MTDH蛋白的阳性表达量高于癌旁正常组织(P<0.05)。乳腺癌组织中MTDH mRNA的阳性表达率高于癌旁正常乳腺组织(P<0.05);乳腺癌组织中MTDH mRNA的阳性表达量高于癌旁正常组织(P<0.05)。结论:乳腺癌的癌变、侵袭、转移可能与MTDH蛋白、mRNA的高表达有关。

关键词: 乳腺肿瘤, MTDH, 蛋白, mRNA

Abstract: Objective Detect the expression of metadherin(MTDH)mRNA and protein and investigate the role of MTDH in the development of breast cancer. Metheds Detect the expression of MTDH protein and mRNA in breast cancer and normal breast tissue by Western bloting and RT-PCR. Analyze the relatives of MTDH protein and clinicothological parameters. Results The positive rate of MTDH protein were significantly higher in cancer tissues than that in normal breast tissues(P<0.05). The expression of MTDH in cancer tissue were correlated with lymphnode metastasis(P<0.05) and were not correlated with TNM stage and differentiation grade. The positive rate of MTDH mRNA were significantly higher in cancer tissues than that in normal breast tissues(P<0.05); The expression of MTDH mRNA were significantly higher in cancer tissues than that in normal breast tissues(P<0.05). Conclusion The carcinogenesis , invasion and metastasis of breast cancer may be related to overexpression of MTDH protein and mRNA.

Key words: Breast tumor, MTDH, Protein, mRNA

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