基础医学与临床 ›› 2011, Vol. 31 ›› Issue (9): 980-983.

• 研究论文 • 上一篇    下一篇

大鼠实验性蛛网膜下腔出血后脑内HSP72的表达

范议方,韩如泉   

  1. 首都医科大学附属北京天坛医院
  • 收稿日期:2010-03-19 修回日期:2010-11-03 出版日期:2011-09-05 发布日期:2011-09-05
  • 通讯作者: 韩如泉 E-mail:hanrq666@yahoo.com.cn
  • 基金资助:
    北京市科委科技新星资助项目(2007B74);教育部留学回国人员科研启动基金;国家95攻关项目基金

Overexpression of HSP72 in cortex and hippocampus after experimental subarachnoid hemorrhage in rats

  • Received:2010-03-19 Revised:2010-11-03 Online:2011-09-05 Published:2011-09-05
  • Contact: Ru-quan HAN E-mail:hanrq666@yahoo.com.cn

摘要: 目的 研究大鼠蛛网膜下腔出血后HSP72在大脑皮层和海马区的表达。方法 36只Wistar大鼠随机分为假手术组(SO)、枕大池一次注血组(SI)及二次注血组(DI) 分别制作蛛网膜下腔出血模型。制备模型成功后取脑行免疫组化染色及Western-blot方法检测海马和大脑皮层区HSP72的表达。结果 大鼠枕大池注血法可复制脑血管痉挛模型;导致海马及大脑皮层区HSP72过度表达;与假手术组相比,枕大池注血组大鼠HSP72的表达显著增加(P<0.05),两注血组间相比差异无显著性(P>0.05)。结论 蛛网膜下腔出血可造成大鼠早期脑损伤,并引起海马及脑皮层HSP72的过度表达。

关键词: 蛛网膜下腔出血 , 脑血管痉挛 , HSP72

Abstract: Objective The 72-kDa heat shock protein (HSP72) is a highly stress-inducible member of a chaperone family of proteins. Overexpressing HSP72 is protective against focal and global cerebral ischemia. We assessed the expression of HSP72 in cortex and hippocampus after experimental subarachnoid hemorrhage in rats. Methods 36 Wistar rats were randomly divided into three groups: single intracisternal blood injection, double intracisternal blood injection and sham operation. The rat in intracisternal blood injection groups were subjected to injection of autologous blood into cisterna magna once or twice on day 0 and day 2 and were sacrificed on days 3, respectively. The expression of HSP72 in hippocampus and cerebral cortex was detected by immunohistochemistry and Western-blot technique. Results HSP72 expression was detected in intracisternal blood injection group by immunohistochemistry. Semiquantitative analysis of HSP72 electrophoretic bands indicated more expression in hippocampus and cerebral cortex in both injection groups compared with the sham group(P<0.01). While there was no significant differences in the two injection groups(P>0.05). Conclusion Subarachnoid hemorrhage produced by single and double intracisternal autogenious blood injection can cause HSP72 overexpression in hippocampus and cerebral cortex.

Key words: Subarachnoid hemorrhage, Cerebral vasospasm, HSP72

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