基础医学与临床 ›› 2011, Vol. 31 ›› Issue (7): 762-766.

• 研究论文 • 上一篇    下一篇

miR-16促进Neuro2a细胞的凋亡

刘畅1,刘伟2,王明2,阴彬1,袁建刚1   

  1. 1. 中国医学科学院基础医学研究所 北京协和医学院基础学院
    2. 中国医学科学院基础医学研究所
  • 收稿日期:2011-05-04 修回日期:2011-05-08 出版日期:2011-07-05 发布日期:2011-07-05
  • 通讯作者: 袁建刚 E-mail:yuanjiangang@pumc.edu.cn
  • 基金资助:
    国家自然科学基金

Overexrpession of miR-16 promotes apoptosis in Neuro2a cells

Chang LIU1,Wei LIU2,Ming WANG2,Bin YIN3,Jian-gang YUAN1   

  1. 1. Institute of Basic Medical Sciences, CAMS & PUMC
    2.
    3. IBMS, CAMS & PUMC
  • Received:2011-05-04 Revised:2011-05-08 Online:2011-07-05 Published:2011-07-05
  • Contact: Jian-gang YUAN E-mail:yuanjiangang@pumc.edu.cn

摘要: 目的 检测过表达miR-16对小鼠Neuro2a细胞周期和凋亡的影响,探讨miR-16在肿瘤发病过程中的可能机制。方法 构建能够在细胞中过表达miR-16的真核表达载体pcDNA3.1-miR-16,利用Real-time PCR验证了表达载体的过表达效应。应用pcDNA3.1-miR-16载体转染Neuro2a细胞,利用流式细胞术和TUNEL法检测Neuro2a细胞周期及凋亡改变情况。 结果 转染过表达pcDNA3.1-miR-16载体后,成熟的miR-16表达与对照组相比明显升高(P<0.05)。在Neuro2a细胞中过表达miR-16后,用流式细胞术检测发现,Neuro2a细胞周期未见明显改变,与对照组相比,凋亡细胞数量有显著增加;进一步通过TUNEL法验证,发现miR-16的过表达能够促进Neuro2a的细胞凋亡。结论 过表达miR-16能够显著促进Neuro2a细胞凋亡而对细胞周期并无影响,提示miR-16可能对肿瘤治疗起到作用。

关键词: miR-16, Neuro2a细胞, 细胞周期, 凋亡, 肿瘤

Abstract: Objective To investigate the role of miR-16 in cell cycle and apoptosis in Neuro2a cells and explore the possible function of miR-16 in tumor. Methods The overexpression plasmid pcDNA3.1-miR-16 of miR-16 was cloned and introduced into Neuro2a cells. Real-time PCR was chosen to test the overexpression of miR-16. Cell cytometry was used to study the role of miR-16 in cell cycle and apoptosis, and apoptosis cells were quantitatively examined by TUNEL. Results After tranfection of pcDNA3.1-miR-16, the mature miR-16 was significantly overexpressed (P<0.05). Cell cytometry results showed that the cell cycle didn’t change after miR-16 overexpression while cell apoptosis were found and confirmed by TUNEL. Conclusion There was obvious apoptosis of Neuro2a cell after overexpression of miR-16, thus, miR-16 might involve in cure of tumor.

Key words: miR-16, Neuro2a cells, cell cycle, apoptosis, tumor

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