基础医学与临床 ›› 2011, Vol. 31 ›› Issue (4): 393-399.

• 研究论文 • 上一篇    下一篇

15d-PGJ2减弱香烟提取物抑制人脐静脉内皮细胞迁移

李炳蔚1,苑晓晨1,2,李宏伟1,修瑞娟1   

  1. 1. 中国医学科学院北京协和医学院微循环研究所
    2.
  • 收稿日期:2011-01-05 修回日期:2011-01-11 出版日期:2011-04-05 发布日期:2011-04-08
  • 通讯作者: 修瑞娟 E-mail:xiurj@yahoo.com.cn
  • 基金资助:
    中国-瑞典合作研究基金

15d-PGJ2 attenuates CSE-induced inhibition of HUVECs migration

  1. 1. Institute of Microcirculation, CAMS & PUMC
    2.
    3. Institute of Microcirculation,CAMS & PUMC
  • Received:2011-01-05 Revised:2011-01-11 Online:2011-04-05 Published:2011-04-08

摘要: 目的 探讨15-脱氧-Δ12, 14-前列腺素J2(15d-PGJ2)对香烟提取物(CSE)抑制人脐静脉内皮细胞(HUVECs)迁移的保护作用。方法 “划痕法”检测HUVECs的迁移。毛细管样成管实验检测HUVECs的血管新生。Western blot检测occludin蛋白表达。结果 与对照组相比,CSE显著抑制HUVECs迁移,使单个视野划痕内细胞数由629±28降低至364±17 (P < 0.01),15d-PGJ2干预可使细胞数回升至546±20 (P < 0.01)。CSE抑制HUVECs血管新生,15d-PGJ2可逆转CSE对HUVECs血管新生的抑制。与对照组相比,CSE使HUVECs occludin蛋白表达量由0.37±0.04降至0.12±0.03 (P < 0.01)。15d-PGJ2干预可使其回调至0.28±0.04 (P < 0.01),但仍低于对照组(P < 0.05)。结论 CSE对HUVEVs迁移的抑制作用可能是通过下调occludin蛋白的表达实现的。15d-PGJ2减弱CSE下调occludin表达而逆转CSE对HUVECs迁移的抑制。

关键词: 香烟提取物, 内皮细胞迁移, 15-脱氧-前列腺素J2

Abstract: Objective To explore the roles of 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) in cigarette smoke extract (CSE)-induced change of human umbilical vein endothelial cells (HUVECs) migration. Methods To determine the migration and angiogenesis of HUVECs with“scratch assay” and capillary-like tube formation assay. To observe the expression of occludin with western blot. Results CSE significantly inhibits the migration of HUVECs. Cell number of wound closure in one visual field decreases from 629±28 in control to 364±17 in CSE group (P < 0.01). 15d-PGJ2 treatment increases the cell number of wound closure from 364±17 in CSE group to 546±20 in 15d-PGJ2+CSE group (P < 0.01). CSE inhibits the angiogenesis of HUVECs. 15d-PGJ2 could attenuate CSE-induced inhibition of HUVECs angiogenesis. The expression of occludin decreases from 0.37±0.04 in control to 0.12±0.03 in CSE group (P < 0.01) and increases to 0.28±0.04 in 15d-PGJ2+CSE group (P < 0.01 compared with CSE group), but still lower than control (P < 0.05). Conclusions CSE may inhibit the migration of HUVECs via down-regulation of occludin. 15d-PGJ2 could attenuate the change of occludin to rescue the HUVECs migration inhibited by CSE.

Key words: Cigarette smoke extract, Endothelial cell migration, 15d-PGJ2

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