基础医学与临床 ›› 2011, Vol. 31 ›› Issue (4): 383-387.

• 研究论文 • 上一篇    下一篇

在乳腺癌干细胞中Hedgehog信号通路相关基因表达增强

陶雅军,毛俊,张晴晴,李连宏   

  1. 大连医科大学
  • 收稿日期:2010-10-25 修回日期:2010-11-23 出版日期:2011-04-05 发布日期:2011-04-08
  • 通讯作者: 李连宏 E-mail:lilianhong@dlmedu.edu.cn
  • 基金资助:
    国家自然科学基金;高等学校博士点基金

Expression Reinforcement of Hedgehog Signaling Genes in Breast Cancer Stem Cells

TAO Ya-jun 1,MAO Jun 2,ZHANG Qing-qing 2,LI Lian-hong 1   

  1. 1. Dalian Medical University
    2.
  • Received:2010-10-25 Revised:2010-11-23 Online:2011-04-05 Published:2011-04-08
  • Contact: LI Lian-hong E-mail:lilianhong@dlmedu.edu.cn

摘要: 目的 了解Hedgehog信号通路在乳腺癌发生发展中的作用。方法 用免疫磁珠法从无血清培养的乳腺癌悬浮细胞中分选CD44+CD24-细胞和非CD44+CD24-细胞,用real-time RT-PCR法检测Hedgehog信号通路主要分子SHH、PTCH1、SMO和GLI1 mRNA在细胞中的表达,用免疫组织化学法检测上述因子在乳腺癌组织中的表达。结果 分选出的CD44+CD24-细胞约占乳腺癌悬浮细胞总数的8.25%,分选出的CD44+CD24-细胞表达干细胞标志蛋白ALDHA1和Oct-4;SHH、PTCH1、SMO和GLI1 mRNA在CD44+CD24-细胞中的表达均高于其在非CD44+CD24-细胞中的表达(P均<0.05);SMO和GLI1蛋白在三阴性乳腺癌的表达均高于非三阴性乳腺癌组织(P均<0.05)。结论 在乳腺癌干细胞CD44+CD24-细胞中Hedgehog信号通路被激活,抑制癌症干细胞中Hedgehog通路的活化可能会降低或阻止乳腺癌的复发及化疗耐受。

关键词: Hedgehog信号通路, 乳腺癌, CD44+CD24-细胞

Abstract: Objective To explore the effect of Hedgehog signaling pathway in breast carcinogenesis. Methods CD44+CD24- cells and non-CD44+CD24-cells were selected from cell suspension in the culture system of a serum-free medium by magnetic activated cell sorting system (MACS), real-time RT-PCR was employed to detect the expression of SHH,PTCH1,SMO and GLI1 mRNA in CD44+CD24- cells and non-CD44+CD24-cells, immunohistochemistry test was applied to study the expressions of SHH,PTCH1,SMO and GLI1 protein in breast cancer. Results CD44+CD24- cells accounted for 8.25% of breast cancer suspension cells and showed the positive expression of ALDH1A1 protein and Oct-4 protein.The expression levels of SHH、PTCH1、SMO、GLI1mRNA in CD44+CD24- cells were higher than those in non-CD44+CD24-cells. SMO and GLI1 expressions were increased in triple-negative breast cancer.Conclusions Hedgehog signaling is activated in breast cancer stem cell CD44+CD24- cells and to stop the recurrence and drug resistance by inhibiting the activation of Hedgehog signaling in breast cancer cells maybe an effective measure.

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