基础医学与临床 ›› 2011, Vol. 31 ›› Issue (12): 1346-1350.

• 研究论文 • 上一篇    下一篇

二十二碳六烯酸降低β淀粉样蛋白25-35致大鼠皮层神经元损伤

王永1,胡为民2,李世芳2,刘丽莉2,张记君2   

  1. 1. 北京康复中心
    2.
  • 收稿日期:2010-10-22 修回日期:2011-05-10 出版日期:2011-12-05 发布日期:2011-12-05
  • 通讯作者: 王永 E-mail:wyxxx2056@sina.com

Ducosahexaenoic acid reduces the cerebral cortical neurons injury induced by β-amyloid-peptide 25-35 in rats

  • Received:2010-10-22 Revised:2011-05-10 Online:2011-12-05 Published:2011-12-05

摘要: 摘要:目的 观察二十二碳六烯酸(DHA)对β淀粉样蛋白25-35(Aβ25-35)致原代培养大鼠皮层神经元损伤的保护作用。方法 原代培养Wistar大鼠皮层神经元,先后给予不同剂量的DHA(20、50、100μmol/L)及Aβ25-35(25μmol/L),用CCK-8比色法观察神经元存活率,用激光共聚焦显微镜观察细胞内游离钙离子浓度。结果:1)与对照组相比,Aβ25-35使细胞存活率明显下降(31± 6 %,n=8, p<0.05);使细胞内游离钙离子浓度明显升高(249±12 %,n=8, p<0.05);2)孵育DHA可降低Aβ25-35引起的神经元存活率明显下降及细胞内游离钙离子浓度升高。结论:Aβ致细胞内钙超载是Aβ产生神经毒作用的一个方面而DHA可部分拮抗Aβ25-35的神经毒作用。

关键词: 二十二碳六烯酸(DHA), β淀粉样蛋白(Aβ25-35), 钙离子, 皮层神经元

Abstract: Abstact: Objective To observe the protection of Ducosahexaenoic acid(DHA)on primary culture cortical neurons exposed to amyloid beta protein 25-35. Methods The newborn Wistar rat cortical neurons were primarily cultured, then treated with DHA of different dose(20、50、100μmol/L) and aggregated Aβ25-35(25μmol/L). CCK-8(Cell Counting Kit-8)staining was used to detect the survival rate of cortical neurons, and LSCM (laser-scanning confocal imaging system)was used to detect the changes of intracellular free calcium concentration in neurons labeled with the fluorescent dye Fluo-3/AM. Results 1)Compared with control group, the survival rate of cortical neurons was decreased in Aβgroup (31± 6 %,n=8, p<0.05), intracellular free calcium concentration in Aβgroup was elevated(249±12 %,n=8, p<0.05); 2)incubation with DHA attenuated the decrease in the survival rate of cortical neurons and the increase in intracellular free calcium concentration induced by amyloid beta protein 25-35. Conclusions:1) Calcium overloading induced by amyloid beta protein 25-35 might be the main neurotoxicity effect ; 2)DHA could partly decrease calcium overloading induced by amyloid beta protein 25-35, which might be the important dmechanism of DHA attenuating the neurotoxicity effect.

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