基础医学与临床 ›› 2011, Vol. 31 ›› Issue (11): 1238-1241.

• 研究论文 • 上一篇    下一篇

PAI-1启动子区4G/5G基因多态性、ACE插入/缺失多态性与脑卒中的相关研究

国雪   

  1. 河北省人民医院
  • 收稿日期:2009-09-24 修回日期:2011-01-17 出版日期:2011-11-05 发布日期:2011-11-02
  • 通讯作者: 国雪 E-mail:guoxue0884@163.com
  • 基金资助:
    省科技发展项目

Study of the relationship between PAI-1 promotor region 4G/5G gene polymorphism,ACE I/D gene polymorphism and cerebral stroke

  • Received:2009-09-24 Revised:2011-01-17 Online:2011-11-05 Published:2011-11-02

摘要: 【摘要】 目的: 探讨纤溶酶原激活物抑制物-1( PAI-1)启动子区基因多态性和血管紧张素转换酶(ACE)插入/缺失多态性与脑卒中的关系。方法:PCR技术检测203例脑卒中患者和139名健康对照者PAI-1基因启动子区4G/5G多态性、ACE基因插入/缺失多态性,同时应用比色法测定血清ACE活性,发色底物法测定PA I - 1活性。结果: 脑梗死(CI)组血浆PAI-1、ACE活性明显高于对照组,脑出血(CH)组血浆PAI-1、ACE活性与对照组相比无明显变化。CI组PAI-I基因4G纯合子、ACE D/I基因DD纯合子比例明显高于对照组,CH组PAI-I基因4G纯合子、ACE D/I基因DD纯合子比例与对照组相比无明显差异。PAI-I基因4G/4G基因型与ACE基因 DD基因型对CI发病可相互协同作用。结论: PAI-1基因4G/4G基因型和ACE基因D/D基因型均可能是CI发病的危险因素,且具有协同作用。

关键词: 脑卒中, 纤溶酶原激活物抑制物, 血管紧张素转换酶, 基因多态性

Abstract: 【Abstract】 Objective To investigate the relationship between gene polymorphism of the plasminogen activator inhibitor-1 (PAI-1) promotor region 4G/5G gene, angiotensin I converting enzyme gene insertion/deletion (I/D) and cerebral stroke. Methods The genotype of 4G/5G allele polymorphism in the PAI-1 promotor region and I/D allele polymorphism in ACE were determined by polymerase chain reaction from leukocytes of 139 normal controls and 203 patients with cerebral stroke. Serum ACE activity was measured by colorimetry, plasma level of PA I-1 activity was determined by spectrophotometric assay. Results The plasma PAI-1 activity (0.769±0.163 AU/mL) and ACE activity in serum (43.42±14.36 U/L) in cerebral ischemia group (CI group) were significantly higher than those in control group (0.652±0.116 AU/mL, 31.28±8.64 U/L, p < 0.01, respectively). The frequency of PAI-1 4G/4G genotype and 4G alleles (43.14% and 62.24%), ACE D/D genotype and D alleles (49.02% and 67.16%) in CI group was significantly higher than those in control group (24.46% and 51.79%; 20.86% and 42.09%, p < 0.05, respectively). However, the difference of these data was not significant between cerebral hemorrhage (CH) and Control group. Furthermore, the effects of PAI-1 4G/4G genotype and ACE D/D genotype on cerebral infarction were synergetic. Conclusion 4G/4G PAI-1 and D/D genotype in ACE may be a susceptible factor to acute cerebral infarction, 4G and D allele homozygous genotype may be the major and synergetic risk factors of acute cerebral infarction.

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