基础医学与临床 ›› 2011, Vol. 31 ›› Issue (1): 32-36.

• 研究论文 • 上一篇    下一篇

小剂量VCR联合Endostar抑制PLA-802细胞系VEGF表达

杨清平,周知,吕波,陶勇,李胜难,罗小红,殷清华   

  1. 重庆市长寿区人民医院
  • 收稿日期:2010-02-24 修回日期:2010-05-10 出版日期:2011-01-05 发布日期:2011-01-05
  • 通讯作者: 杨清平

Insight into the effect of the combination of small dosage of VCR and Endostar on the expression of VEGF in PLA-802 cell line

YANG Qing-ping 1, ZHOU Zhi 2,LU Bo 2,TAO Yong 2,Li Sheng-nan 2,Luo Xiao-hong 2,Yin Qing-hua 2   

  1. 1. People’s Hospital of Changshou District, Chongqing2.
  • Received:2010-02-24 Revised:2010-05-10 Online:2011-01-05 Published:2011-01-05
  • Contact: YANG Qing-ping

摘要: 目的 探讨小剂量长春新碱(VCR)联合恩度(Endostar,Endo)对横纹肌肉瘤细胞生长的影响及其机制。方法 体外培养PLA-802细胞株,用小剂量Endo、VCR单独或联合处理细胞。用MTT和流式细胞仪检测细胞的生长和细胞周期;用RT-PCR和Western blot 检测药物处理后细胞内VEGF mRNA和蛋白水平;用ELISA检测上清液中VEGF水平。结果 单独应用VCR(0.5和2.0 μg/ml)和单独应用Endo(2.0μg/ml)均能够抑制PLA-802细胞的增殖,并促进细胞的凋亡,抑制细胞内VEGF的表达和生成。而两者联合应用时,上述作用得到明显增强。结论 小剂量VCR与Endo联合应用协同抑制PLA-802细胞中VEGF的生成和表达和诱导细胞凋亡。

关键词: 恩度, 长春新碱, PLA-802细胞, 血管内皮生长因子

Abstract: Objective To explore the effect of the combination of small dosage of VCR and Endostar on rhabdomyosarcoma cells and its mechanisms. Methods PLA-802 was cultured and treated with small dosage of VCR or Endostar singly or together. MTT was used to study the cells proliferation, and flow cytometry was for the cell cycle. While, RT-PCR and Western blot were for the expression of VEGF mRNA and protein levels. ELISA was for the generation of VEGF in supernatant. Results VCR (0.5 and 2.0μg/ml) or Endo (2.0 μg/ml) used singly inhibited the cell proliferation and promoted the apoptosis, and inhibited the expression and generation of VEGF. When the cells were treated with VCR and Endo together, the inhibitory effects of them were significantly strengthened. Conclusion The combination of VCR and Endostar could synergistically inhibit the expression and generation of VEGF and inducing the apoptosis in PLA-802 cells.