基础医学与临床 ›› 2010, Vol. 30 ›› Issue (7): 763-766.

• 研究论文 • 上一篇    下一篇

IL-10基因多态性与特应性皮炎的相关性

许银姬 佟金平 王惠娟   

  1. 大连大学附属新华医院
  • 收稿日期:2009-10-26 修回日期:2010-01-08 出版日期:2010-07-05 发布日期:2010-07-05
  • 通讯作者: 许银姬

Association of IL-10 Gene Polymorphism with Atopic Dermatitis

Yin-ji XU, Jin-ping TONG, Hui-juan WANG   

  • Received:2009-10-26 Revised:2010-01-08 Online:2010-07-05 Published:2010-07-05
  • Contact: Yin-ji XU,

摘要: 目的 研究白细胞介素-10(IL-10)基因启动子区域的单核苷酸多态性与特应性皮炎发生的相关性。方法 门诊特应性皮炎患儿174名和健康儿童130名,利用Taqman方法分析IL-10基因启动子区域 -1082(G/A), -819(C/T), -592(C/A) 单核苷酸多态性和单核苷酸组合成的单倍型和基因型。分析特定基因型保留和血液中嗜酸性粒细胞总数或血浆中IgE浓度之间的关联性。结果 健康组儿童ACC单倍型频率显著高于特应性皮炎儿童(p﹤0.05)。而血液中嗜酸性粒细胞总数和血浆中IgE浓度,ATA/ATA基因型的儿童显著高于ATA/ACC基因型儿童(p﹤0.05)。结论 IL-10基因启动子区域的单核苷酸多态性与特应性皮炎发生有关。

关键词: 白细胞介素-10, 基因多态性, 特应性皮炎, 单元型, 基因型

Abstract: Objective To study the association of the interleukin-10(IL-10) haplotype polymorphism and the Atopic Dermatitis. Methods 174 Atopic Dermatitis children and 130 healthy children were tested, The frequency of three single nucleotide polymorphisms(SNPs) at positions-1082(A/G), -819(C/T), -592(A/C) and corresponding haplotypes in the promoter region of the IL-10 gene were analysed using Taqman method. The data were assessed for correlations with the eosinophil count and total serum IgE concentration. Results The frequency of IL-10 haplotype ACC in healthy children was higher than that in Atopic Dermatitis children (P﹤0.05) . Meanwhile medians of serum IgE level and total eosinophil count in Atopic Dermatitis children with ATA/ATA genotype were higher than those with ATA/ACC (P﹤0.05) . Conclusion IL-10 promotor polymorphism may be associated with the development of Atopic Dermatitis.

Key words: IL-10, Gene polymorphism, Atopy, Haplotype, Genotype