基础医学与临床 ›› 2010, Vol. 30 ›› Issue (5): 471-475.

• 研究论文 • 上一篇    下一篇

补体介导Fcα/μ受体对人肾小球系膜细胞的杀伤

沈濂 王晓燕 赵青 张伟   

  1. 中国医学科学院 基础医学研究所 中国医学科学院 基础医学研究所 中国医学科学院基础医学研究所
  • 收稿日期:2010-01-18 修回日期:2010-01-25 出版日期:2010-05-05 发布日期:2010-05-05
  • 通讯作者: 张伟

Complement mediated killing of human glomerular mesangial cell by Fcα/μ Receptor

Lian SHEN, Xiao-yan WANG, Qing ZHAO, Wei ZHANG   

  1. IBMS, CAMS & PUMC IBMS, CAMS & PUMC
  • Received:2010-01-18 Revised:2010-01-25 Online:2010-05-05 Published:2010-05-05
  • Contact: Wei ZHANG

摘要: 目的 研究补体能否经由Fcα/μR介导,杀伤人肾小球系膜细胞。方法 藉脂质体将带有Fcα/μR cDNA的质粒pcDNA3.1-Fcα/μR转染入人肾小球系膜细胞(NHMC),用Western blot检测Fcα/μR在NHMC细胞的表达,激光共聚焦显微术检测Fcα/μR在细胞膜的表达。用流式细胞术与激光共聚焦显微术检测所制备的IgM型免疫复合物(IgM-IC)与膜表达Fcα/μR的NHMC细胞的结合,用台盼蓝染色检测补体介导的细胞杀伤作用。结果 成功制备了胞膜表达 Fcα/μR的NHMC细胞,IgM与IgM-IC能经由Fcα/μR结合于NHMC细胞。在补体作用下,结合有IgM-IC的pcDNA3.1-Fcα/μR转染细胞死亡率显著高于对照组野生型细胞,空载质粒转染细胞及无IgM-IC结合的pcDNA3.1-Fcα/μR转染细胞(P 0.001)。结论 胞膜表达Fcα/μR并结合有IgM-IC的人肾小球系膜细胞能为补体所杀伤。

关键词: Fcα/μR, IgM, 免疫复合物, 补体, 肾小球系膜细胞

Abstract: Objective To study whether Fcα/μR can mediate complement killing of human glomerular mesangial cells. Methods The Fcα/μR cDNA contained plasmid, pcDNA3.1-Fcα/μR was transfected into a human glomerular mesangial cell line, NHMC. Fcα/μR expression was detected by Western blotting and laser scanning confocal microscopic analysis. Binding of IgM-immune complexes (IgM-IC) to the Fcα/μR on cell membrane was detected by flowcytometry and laser scanning confocal microscopyic analysis. Killing of cells by complement was analyzed by Trypan blue exclusion assay. Results NHMC cells transfected with Fcα/μR could bind IgM and IgM-IC. After treatment with complement, added IgM-IC, the death rate of pcDNA3.1-Fcα/μR transfected cell was significant higher than the control groups of wild type cell, pcDNA3.1 transfected cell and the pcDNA3.1-Fcα/μR transfected cell without IgM-IC. Conclusion IgM-IC can be bound by Fcα/μR expressed NHMC cells and mediate complement killing of the cells.

Key words: Fcα/μR, IgM, Immune complexes, complement, glomerular mesangial cell

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