基础医学与临床 ›› 2010, Vol. 30 ›› Issue (3): 297-302.

• 研究论文 • 上一篇    下一篇

60株鲍曼不动杆菌耐药基因携带情况分析

王玉平 宋艳荣 李继红 刘爱翔   

  1. 邢台医学高等专科学校 邢台市人民医院
  • 收稿日期:2009-07-14 修回日期:2009-09-27 出版日期:2010-03-05 发布日期:2011-05-04
  • 通讯作者: 王玉平

Analysis of drug-resistant genes of 60 Acinetobacter baumannii isolates

Yu-ping WANG, Yan-rong SONG, Ji-hong LI, Ai-xiang LIU   

  1. XingTai Medical College Xing Tai People's Hospital
  • Received:2009-07-14 Revised:2009-09-27 Online:2010-03-05 Published:2011-05-04
  • Contact: Yu-ping WANG,

摘要: 目的 研究临床分离的60株鲍曼不动杆菌耐药谱及Ⅰ型整合子和β-内酰胺酶等基因携带情况。方法 用微量肉汤稀释法测定16种临床常用抗菌药物的最小抑菌浓度;PCR检测β-内酰胺酶、Ⅰ型整合子和外排泵基因,对阳性基因进行序列分析。结果 60株菌中,多重耐药株53株,占88.3%;6株携带OXA-23基因,均对包括碳青霉烯在内的5类以上抗菌药耐药,并具有高耐药特性;38株携带PER-1基因,对头孢菌素类耐药率显著高于PER-1基因阴性菌株(P<0.01);45株检出Ⅰ型整合子结构基因,多重耐药率明显高于Ⅰ型整合子阴性菌株(P<0.01);Ⅰ型整合子和PER-1基因同时阳性25株,与7株两者同为阴性菌株相比,多重耐药率增高(P<0.01),但耐药程度无显著差别。结论 Ⅰ型整合子基因及β-内酰胺酶类基因的作用是导致鲍曼不动杆菌多重耐药的重要原因;OXA-23基因阳性菌株多为泛耐药和高耐药株,有必要采取有效措施控制其传播。

关键词: 鲍曼不动杆菌, 多重耐药, 分子机制

Abstract: Objective To investigate β-lactamase gene and class 1 integron gene of 60 clinical Acinetobacter baumannii isolates. Methods The minimal inhibitory concentrations (MICs)for 16 antibiotics widely used in clinic were determined using the standard broth microdilution method. The β-lactamase gene, class 1 integron gene and adeB gene were determined by PCR and were then sequenced. Results Fifty-three strains of the 60 A. baumanii isolates were multi-drug resistant. OXA-23 gene was detected positive in six A. baumanii isolates, which were all resistant to more than five antimicrobial agents including carbapenem and showed high resistance to many antibiotics. The 38 strains that carried PER-1 gene showed higher resistance to cephalosporins than those without this gene (P<0.01). Class 1 integron gene was positive in 45 strains, which exhibited significantly higher multiple resistance than those without this gene (P<0.01). The 25 strains carrying both class 1 integron and PER-1 genes had a markedly higher multiple resistance (P<0.01), but not in resistant level, compared to the 7 strains without these two genes. Conclusion Class 1 integron and β-lactamase gene may be the causes of multidrug-resistance of A. baumanii. The strains carrying OXA-23 gene always showed multiple and high resistance to several antibiotics, so effective measures must be taken to control the epidemic of these strains.

Key words: Acinetobacter baumannii, Multidrug-resistance, molecular mechanism