基础医学与临床 ›› 2009, Vol. 29 ›› Issue (8): 831-834.

• 研究论文 • 上一篇    下一篇

幽门螺杆菌及其L型与表面蛋白-D的结合力的检测与分析

陈峥宏 赵亮 王菲   

  1. 贵阳医学院 贵阳医学院 贵阳医学院
  • 收稿日期:2008-09-11 修回日期:2009-02-25 出版日期:2009-08-20 发布日期:2009-08-20
  • 通讯作者: 陈峥宏

Interactions of Helicobacter pylori and its' L-forms with surfactant protein D

Zheng-hong CHEN, Liang ZHAO, Fei WANG   

  1. Guiyang Medical College
  • Received:2008-09-11 Revised:2009-02-25 Online:2009-08-20 Published:2009-08-20
  • Contact: Zheng-hong CHEN,

摘要: 目的 研究幽门螺杆菌(Hp)及其L型与表面活性蛋白-D(SP-D)的结合力,探讨SP-D在Hp感染和免疫中的意义。方法 用Tris-HCl-EDTA法和麦芽糖-琼脂糖亲和柱层析法制备大鼠肺SP-D。用Hp与SP-D进行玻片和试管凝集试验,加入SP-D家兔抗血清进行凝集抑制试验。以抗生素诱导法获得Hp L型,通过Hp 16SrDNA聚合酶链反应和扩增产物的序列测定鉴定L型菌种。以玻片凝集试验检测Hp L型与SP-D的结合力。结果Hp NCTC11637可与SP-D发生肉眼可见的玻片凝集,而Hp SS1、NCTC11639及L型与SP-D未发生凝集。Hp NCTC11637与SP-D在反应45min时出现明显凝集。SP-D抗血清抑制Hp与SP-D的凝集。结论 Hp不同菌株与SP-D的结合力不同;发生细胞壁缺陷变异后Hp与SP-D不再发生明显的凝集。

关键词: 幽门螺杆菌, 细胞壁缺陷, 表面活性蛋白-D

Abstract: Objective To explore the ability of combination of Helicobacter pylori (Hp) and its' L-form with Surfactant protein D(SP-D), and the function of SP-D in Hp infection and immunity. Methods SP-D were extracted from the lung of rats by Tris-HCl-EDTA and maltose-agarose affinity column. The combination of Hp and SP-D were detected by slide and tube agglutination tests, and the agglutination inhibition with SP-D immune sera was carried out. The L-form of Hp was induced by Ceftriaxon sodrum and identified by PCR and sequencing of the 16SrDNA fragment.The combination of SP-D and the L-form was detected by the slide agglutination test. Results Agglutination of Hp NCTC11637 and SP-D could be observed on slide, while which of NCTC11639, SS1 and the L-form couldn't. Agglutination could be detected 45 minutes after reaction and could be inhibited by SP-D immune sera. Conclusions The ability of combination with SP-D could be different between strains of Hp.The combination could be weakened or vanished when Hp lost the cell wall.

Key words: Helicobacter pylori, cell wall deficient, Surfactant protein D

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