基础医学与临床 ›› 2009, Vol. 29 ›› Issue (8): 801-805.

• 研究论文 • 上一篇    下一篇

颞叶癫痫患者颞叶皮层和海马组织内多药耐药相关蛋白1 和胶质原纤维酸性蛋白共表达

杨志军 王春枝 王娆 卢洪流 徐如祥 李安民 梁树立   

  1. 北京军区总医院神经外科 南方医科大学珠江医院
  • 收稿日期:2008-06-17 修回日期:2008-10-23 出版日期:2009-08-20 发布日期:2009-08-20
  • 通讯作者: 徐如祥

Co-expression of multiple drug resistance gene protein 1 and glial fibrillary acidic protein in patients with temporal lobe epilepsy in temporal lobe and hippocampus

Zhi-jun YANG, Chun-zhi WANG, Rao WANG, Hong-liu LU, Ru-xiang XU, An-min LI, Shu-li LIANG   

  1. Department of Neurosurgery, General Hospital of Beijing Military District of Chinese PL Zhujiang Hospital, Southern Medical University
  • Received:2008-06-17 Revised:2008-10-23 Online:2009-08-20 Published:2009-08-20
  • Contact: Ru-xiang XU,

摘要: 目的 观察颞叶癫痫病人多耐药基因1(MDR1)、胶质原纤维酸性蛋白(GFAP)和神经元特异性烯醇化酶(NSE)在颞叶和海马组织内的表达。方法 癫痫组样本来自12例颞叶癫痫病例的手术切除标本,对照组为4例非癫痫病的尸检脑组织。应用双重免疫荧光组织化学方法结合激光共聚焦显微镜技术显示MDR1、GFAP和NSE在颞叶和海马组织内的表达。结果 对照组颞叶皮质和海马齿状回内均可见到许多GFAP表达阳性的星形胶质细胞和NSE表达阳性的神经元,未见到表达MDR1的细胞。癫痫组颞叶皮质和海马齿状回内GFAP阳性星形胶质细胞高于对照组。颞叶皮层和海马组织内可见星形胶质细胞MDR1 和GFAP 共表达现象。结论 颞叶难治性癫痫可能与星形胶质细胞的多药耐药性有关。

关键词: 颞叶癫痫, 海马, 多药耐药基因蛋白, 胶质原纤维酸性蛋白

Abstract: Objective To detect the expression of multiple drug resistance gene protein 1(MDR-1), neuron-specific endase (NSE), and glial fibrillary acidic protein (GFAP) in the temporal lobe and hippocampal dentate gyrus of patients with temporal lobe epilepsy.Methods In epileptic group, the brain tissues including dentate gyrus and temporal lobe were obtained by operation in 12 patients with temporal lobe epilepsy. In control group, the brain tissues were obtained from 4 dead patients underwent autopsy. By means of anti-MDR1 and GFAP or NSE double immunofluorescence histochemical staining method combining with confocal laser-scanning microscopic technique, the distribution of MDR1 or NSE positive cells and reactive astrocytes were observed in the temporal lobes and hippocampus.Results In control group, many GFAP positive astrocytes and NSE positive neuron could be found in the cortex of temporal lobe and dentate gyrus of hippocampus. No MDR1 positive cells could be observed in above-mentioned areas.In epileptic group,GFAP positive astrocytes within the cortex of temporal lobe and dentate gyrus of hippocampus increased markedly. GFAP and MDR1 could co-express in the same astrocyte in the cortex of temporal lobe and dentate gyrus of hippocampus. Conclusion It was suggested that multidrug resistance gene of astrocytes might play an important role in the pathogenesis of intractable epilepsy.

Key words: Temporal lobe epilepsy, Hippocampus, Multiple drug resistance gene protein, Glial fibrillary acidic protein