特发性男性不育患者精浆蛋白质分析

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (7): 688-690.

特发性男性不育患者精浆蛋白质分析

任绪义周敏兰张欣宗刘洋虞闰六

杭州迪安医学检验中心杭州迪安医学检验中心

收稿日期:2008-02-25 修回日期:2008-10-22 出版日期:2009-07-20 发布日期:2009-07-20
通讯作者: 虞闰六

Analysis of Proteomics of Seminal Plasma from Idiopathic Male Infertility

Xu-yi REN, Min-lan ZHOU, Xin-zong ZHANG, Yang LIU, Run-liu YU

Hangzhou D.A.Medical Laboratory Hangzhou D.A.Medical Laboratory

Received:2008-02-25 Revised:2008-10-22 Online:2009-07-20 Published:2009-07-20
Contact: Run-liu YU

摘要/Abstract

摘要： 目的寻找诊断特发性男性不育的生物标记分子。方法应用二维电泳与质谱技术（MS+MS/MS）分析特发性男性不育患者与正常对照组精浆蛋白质组成，寻找差异表达蛋白质。结果特发性男性不育患者较正常对照组精浆中血清白蛋白和前列腺酸性磷酸酶减少，而组织蛋白酶B和锌α2糖蛋白增加。结论特发性男性不育可能与精子获能受阻及精液免疫功能紊乱有关。

关键词: 特发性男性不育, 精浆, 蛋白质组学

Abstract: Objective To discover biological markers. Methods Two-dimensional electrophoresis and Mass-Spectrum techniques (MS+MS/MS) were used for analyzing the seminal plasma from idiopathic male infertility and control. Results Serum albumin and prostatic acid phosphatase were reduced in seminal plasma from idiopathic male infertility rather than the control, while Cathepsin B and Zn-alpha-2-glycoprotein were increased. Conclusion Idiopathic male infertility possibly was associated with disorder of sperm capacitation and seminal immune function.

Key words: Idiopathic male infertility, Seminal plasma, Proteomics

任绪义周敏兰张欣宗刘洋虞闰六. 特发性男性不育患者精浆蛋白质分析[J]. 基础医学与临床, 2009, 29(7): 688-690.

Xu-yi REN; Min-lan ZHOU; Xin-zong ZHANG; Yang LIU; Run-liu YU. Analysis of Proteomics of Seminal Plasma from Idiopathic Male Infertility[J]. Basic & Clinical Medicine, 2009, 29(7): 688-690.

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