基础医学与临床 ›› 2009, Vol. 29 ›› Issue (5): 522-526.

• 研究论文 • 上一篇    下一篇

Hedgehog信号传导通路在乳腺癌中表达增强

黄海林 胡志前 王为民 王毅 蔡清萍 王强   

  1. 上海第二军医大学附属长征医院普外科 上海第二军医大学附属长征医院普外科 上海第二军医大学附属长征医院 上海第二军医大学附属长征医院 上海第二军医大学附属长征医院 上海第二军医大学附属长征医院
  • 收稿日期:2007-11-01 修回日期:2008-09-28 出版日期:2009-05-25 发布日期:2009-05-25
  • 通讯作者: 胡志前

Expression reinforcement of Hedgehog Signaling Pathway in Patients with Breast Cancer

Hai-lin HUANG, Zhi-qing HU, Wei-min WANG, Yi WANG, Qing-ping CAI, Qiang WANG   

  1. Department of General Surgery,Changzheng Hospital,The Second Military Medical University Department of General Surgery,Changzheng Hospital,The Second Military Medical University Department of General Surgery,Changzheng Hospital,The Second Military Medical University Department of General Surgery,Changzheng Hospital,The Second Military Medical University Department of General Surgery,Changzheng Hospital,The Second Military Medical University Department of General Surgery,Changzheng Hospital,The Second Military Medical University
  • Received:2007-11-01 Revised:2008-09-28 Online:2009-05-25 Published:2009-05-25
  • Contact: Zhi-qing HU,

摘要: 目的:研究乳腺癌细胞中,Hedgehog信号传导通路是否表现为持续的激活状态。方法:用免疫组化方法在64例乳癌标本中检测决定Hedgehog信号传导通路的组成部分,包括Shh,patched1和Gli1,SMO的表达。结果:在64例肿瘤样本中,强阳性表达Shh ,Ptch1和Glil,Smo的分别为64(100%),61(95.3%),62(96.9%),和61(95.3%),相反,各自对应相邻的正常乳腺上皮在可探测到的水平上不表达或低表达的这些蛋白,乳腺癌中的Hh通路的激活状态是普遍的,与病人的淋巴结转移无关,与癌肿的大小也无关。但与乳腺癌的特殊的病理类型可能有关。所有的64例肿瘤标本与周围正常组织相比都表现为Gli1强阳性染色,核染色Glil阳性/所有的肿瘤细胞的百分比从2%~95%不等,中位数为40.87%。Gli的核染色与雌激素受体阳性相关(P<0.05),与孕激素受体阳性无关。结论:Hedgehog(Hh)信号传导通路可以作为潜在的乳腺癌治疗的新颖靶点。

Abstract: Objective To investigate whether the Hedgehog (Hh) signaling pathway is activated in human breast cancer. Method we determined the expression of the components, including Sonic Hh, Patched1, and Gli1,Smo of the Hh pathway by immunohistochemical staining in a series of 64 human breast carcinomas. Result In the 64 tumor specimens,there are 64(100%),61(95.3%),62(96.9%),and 61(95.3%)highly express protein Shh ,Patched1 and Glil,Smo respectively,while in the adjacent nomal mammary tissue, correspondingly,those protein are not or weakly express at can detect level.So,the Hedgehog (Hh) signaling pathway is activated in human breast cancer commonly,which has no relative to lymphoid node metabasis,or size of carcinomor, but maybe connect with some special histologic type. All of 64 tumors displayed staining of high intensity for Gli1 when compared with adjacent normal tissue. The ratio of nuclear positive staining of Gli1/all the tumor cell is range from 2%-95%,median at 40.87%. The nuclear staining of Gli1 correlated with expression of estrogen receptor (P<0.05),but has no relative to progesterone receptor。Conclusion These data indicate that the Hh pathway is a new candidate for therapeutic target of breast cancer.