基础医学与临床 ›› 2009, Vol. 29 ›› Issue (5): 475-478.

• 研究论文 • 上一篇    下一篇

乙肝病毒X蛋白对人肝细胞内β-catenin定位及转录活性的影响

丁浩 何柳兴 冯涛   

  1. 重庆医药高等专科学校 成都大学医护学院 重庆医科大学
  • 收稿日期:2008-02-25 修回日期:2008-08-28 出版日期:2009-05-25 发布日期:2009-05-25
  • 通讯作者: 冯涛

Hepatitis B virus X protein effect on nuclear localization and transcription of β-catenin in human liver cell

Hao DING, Liu-xing HE, Tao FENG   

  1. Chongqing Pharmaceutical College Nursing Institute, Chengdu University Chongqing Medical University
  • Received:2008-02-25 Revised:2008-08-28 Online:2009-05-25 Published:2009-05-25
  • Contact: Tao FENG

摘要: 目的 研究HBx对Wnt/Wingless信号传导通路中主要信号分子 -catenin的影响,探讨HBV相关性HCC的发生机制。方法 Ad-HBx转染人肝细胞系L02细胞,免疫细胞化学和Luciferase检测细胞中?-catenin细胞定位及活性变化。结果 免疫细胞化学:转染重组HBx腺病毒前L02细胞中β-catenin仅在细胞质有少量表达,转染后胞质表达明显增强,并出现胞核的大量积聚;Luciferase检测:实验组TOP荧光素酶活性与对照组及空白组相比明显增强,为阴性对照的11倍(P<0.05),Ad- HBx感染后24和36h 的TOP荧光素酶活性无显著差异。结论 Ad-HBx重组腺病毒在体外能有效转染人肝细胞系L02细胞,受感染细胞能有效表达HBx蛋白,HBx影响了人肝细胞系L02细胞中β-catenin的细胞定位及转录活性。

关键词: Wnt/β-catenin信号转导通路, HBX, β-catenin, 腺病毒载体, 肝细胞肝癌

Abstract: Objective To investigate the interaction between HBx protein and β-catenin, illuminate the molecular mechanism in HBV-associated hepatocellular carcinoma Methods Iimmunocytochemistry and Luciferase Assay methods were used to detect the endocellular location and transcriptional activity of β-catenin in normal liver cell line L02 infected by Ad-HBx. Results Iimmunocytochemistry: there was markedly increased expression of β-catenin in cytoplasm and nucleus. Luciferase Assay: Ad-HBx led to significantly increase of TCF-4-dependent transcription, the TOP count of Ad-GFP and Ad-HBx-GFP was 1.4 fold and 11fold compared to the negative control respectively(P<0.05). Conclusions There is the expression of HBx protein in human normal liver cell line (L02) with infected Ad-HBx. This data suggests that ectopic expression of HBx results in the accumulation ofβ-catenin in the cytoplasm and subsequent nuclear localization and increases TCF-4-dependent transcription in L02 cells. HBx protein is essential for the activation of wnt/β-catenin signaling by increasing the expression of β-catenin in normal liver cells.

Key words: Wnt/β-catenin signaling pathway, HBX protein, beta-catenin, adenoviruses, hepatocellular carcinoma

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