基础医学与临床 ›› 2009, Vol. 29 ›› Issue (10): 1083-1086.

• 研究论文 • 上一篇    下一篇

糖尿病大鼠胃平滑肌细胞凋亡与线粒体膜电位改变

赵宏贤 陈霞   

  1. 泸州医学院组胚教研室 泸州医学院附属医院
  • 收稿日期:2008-11-21 修回日期:2009-01-21 出版日期:2009-10-20 发布日期:2009-10-20
  • 通讯作者: 陈霞

Apoptosis and mitochondrial membrane potential change of gastric smooth muscle cells in diabetic rats

Hong-xian ZHAO, Xia CHEN   

  1. Department of Histology and Embryology, Luzhou Medical College Affiliated Hospital, Luzhou Medical College
  • Received:2008-11-21 Revised:2009-01-21 Online:2009-10-20 Published:2009-10-20
  • Contact: Xia CHEN

摘要: 目的 揭示糖尿病胃轻瘫(DGP)发病机制 方法 SD大鼠随机分为对照组和模型组,造模10周后,模型检测;流式细胞术测细胞凋亡与 m;免疫组化测CYT C。结果 与对照组相比:(1)模型组造模后均出现多尿、多饮、多食症状,体重减轻;血糖浓度显著增高 (P<0.01);胃内色素残留率显著降低(P<0.01)。(2)模型组胃平滑肌细胞凋亡率显著增高(P<0.01), m显著降低(P<0.01), 胞质CYT C显著增加。结论 线粒体介导了糖尿病大鼠胃平滑肌细胞凋亡,后者在糖尿病胃轻瘫发病机制中扮演了一定角色。

关键词: 糖尿病, 胃轻瘫, 细胞凋亡, 线粒体膜电位

Abstract: Objective To investigate the associated mechanism of gastroparesis in Diabetic Rats. Methods SD rats were randomly divided into control group and diabetic group. 10 weeks later, gastroparesis model was detected. The apoptosis rate and mitochondrial membrane potential of the gastric smooth muscle cells were detected by flow cytometry.Cytochrome C was detected by immunohistochemistry. Results Compared with rats in control group: ①Typical symptoms of diabetes mellitus appeared; The rate of gastric residual pigment was significantly higher(P<0.01). ②Apoptosis rate was significantly higher(P<0.01); Mitochondrial membrane potential was significantly lower(P<0.01); Cytochrome C was significantly higher(P<0.05). Conclusions Mitochondrion contributes to smooth muscle cell apoptosis ,which paly a partial role in diabetic gastroparesis.

Key words: Diabetes, Gastroparesis, Apoptosis, Mitochondrial membrane potential

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