基础医学与临床 ›› 2009, Vol. 29 ›› Issue (10): 1070-1074.

• 研究论文 • 上一篇    下一篇

脂肪因子对急性肝损伤小鼠肝细胞凋亡的作用

尹洪竹 赵彩彦 刘英晖 宋艳改   

  1. 河北医科大学第三医院感染科 河北医科大学第三医院感染科
  • 收稿日期:2008-10-30 修回日期:2009-01-13 出版日期:2009-10-20 发布日期:2009-10-20

Role of adipokines on hepatocellular apoptosis in mice

Hong-zhu YIN, Cai-yan ZHAO, Ying-hui LIU, Yan-gai SONG   

  • Received:2008-10-30 Revised:2009-01-13 Online:2009-10-20 Published:2009-10-20

摘要: 目的 探讨脂肪因子--脂联素和瘦素对急性肝损伤小鼠肝细胞凋亡的干预作用。方法 雄性BALB/c小鼠26只随机分为4组:刀豆蛋白A(ConA)组8只:静脉注射ConA;脂联素组5只:在静脉注射ConA前腹腔内注射脂联素;瘦素组5只:在静脉注射ConA前腹腔内注射瘦素;对照组8只:静脉注射生理盐水。注射ConA8h后处死小鼠,HE染色观察肝组织的炎症程度,TUNEL法和流式细胞术检测肝细胞凋亡率。结果 与ConA组比较,脂联素组肝组织炎症程度明显减轻,血清ALT水平和肝细胞凋亡率均降低(p<0.01),瘦素组肝细胞凋亡率升高(p<0.01),而肝组织炎症变化及血清ALT水平两组无差异。结论 脂联素可抑制肝细胞凋亡、减轻急性肝损伤,而瘦素则促进肝细胞凋亡,对炎症无明显影响。

Abstract: Objective To investigate the interference role that adipokines----adiponectin and leptin exert to hepatocellular apoptosis in mice suffering acute liver injury induced by Concanavalin A (ConA). Methods In the present study, 26 male BALB/c mice were divided to four groups randomly: ConA group( 8 mice): ConA were intravenous (i.v.) injected; adiponectin group(5 mice): adiponectin were intraperitoneal (i.p.) injected before ConA i.v. injected; leptin group(5 mice): leptin were i.p. injected before ConA i.v. injected; control group(8 mice): normal saline was i.v. injected. Mice were sacrificed 8 hours after ConA-treatment for observing hepatic pathology and hepatocellular apoptotic rate. Results Comparison to ConA group ,the mice in adiponectin group developed reduced hepatic injury and lower ALT level and hepatocellular apoptotic rate(p<0.01), but those in leptin group developed heightened hepatocellular apoptotic rate(p<0.01)and similar ALT levels. Conclusions Adiponectin might inhibit hepatocellular apoptosis and control hepatic inflammation, but leptin improved hepatocellular apoptosis and had no effection on ALT levels induced by ConA .