基础医学与临床 ›› 2009, Vol. 29 ›› Issue (10): 1065-1069.

• 研究论文 • 上一篇    下一篇

血糖波动对糖尿病大鼠海马神经元凋亡相关蛋白表达的影响

李伟 孙娟 殷松楼 李秀芹   

  1. 徐州医学院附属医院 徐州医学院附属医院
  • 收稿日期:2008-10-06 修回日期:2008-12-26 出版日期:2009-10-20 发布日期:2009-10-20
  • 通讯作者: 孙娟

Effects of fluctuant high blood glucose on expression of apoptosis-related genes in hippocampus of diabetic rats

Wei LI, Juan SUN, Song-lou YIN, Xiu-qin LI   

  1. Affiliated Hospital of Xuzhou Medical College Affiliated Hospital of Xuzhou Medical College
  • Received:2008-10-06 Revised:2008-12-26 Online:2009-10-20 Published:2009-10-20
  • Contact: Juan SUN,

摘要: 目的 观察糖尿病大鼠在血糖波动状态下海马神经元的病理形态学改变及BCL-2、BAX的异常表达,探讨血糖波动对糖尿病大鼠海马神经元的影响。方法 用链脲佐菌素(STZ) 诱导,间断短效胰岛素应用制备糖尿病血糖波动模型。12周后,用免疫组化法检测海马神经元凋亡蛋白BCL-2、BAX 的表达,分别在光镜、透射电镜下观察各组海马神经元病理形态学改变及超微结构的变化。结果DM组BCL-2的表达较N组显著下降,BAX显著升高(P<0.01),出现锥体细胞退变的典型病理改变。RDM组BCL-2的表达较DM组进一步下降,BAX进一步升高,差异有统计学意义 (P<0.05),病理改变较DM组进一步加重。结论 糖尿病大鼠血糖波动可明显加速海马CA1区神经元凋亡,BCL-2、BAX的异常表达可能是糖尿病大鼠海马神经元损伤机制之一。

关键词: Bcl-2, Bax, 血糖波动, 糖尿病, 海马, 凋亡

Abstract: Objective To investigate the effect of fluctuant high blood glucose on observing the changes of pathology and BCL-2 ,BAX expression in neurons of the hippocampus from diabetic rats. Methods The diabetic rat models were produced by injection with l% streptozotocin(STZ),followed by insulin administration . regular insulin were used to set up fluctuant high glucose group model. After 12 experimental weeks, the express of Fas protein in hippocampus was studied by immunohistochemistry , changes of pathology and ultrastructure were observed by using light microscopes and electron microscopes in three groups .Results:Compared with those in N group,the expression of BCL-2 in pyramidal cells of diabetic rat's hippocampus was lower,while that of BAX was higher (P<0.01).The pathologic changes of retrogression were significantly serious.The pathologic changes in RDM group were more serious in fluctuant high blood glucose rats group (P<0.05) Conclusion Fluctuant high blood glucose induces more apoptosis in hippocampal neurons cells .The expression of BCL-2, BAX may be one of the mechanism that hippocampus neuron injury in diabetic rats was aggravated.

Key words: Bcl-2, Bax, fluctuant high blood, diabetes mellitus, hippocampus, apoptosis