基础医学与临床 ›› 2009, Vol. 29 ›› Issue (1): 10-13.

• 研究论文 • 上一篇    下一篇

Sca1+间充质干细胞支持造血干细胞向树突细胞分化

陈媛 刘星霞 赵春华   

  1. 中国医学科学院 基础医学研究所 组织工程中心 中国医学科学院 基础医学研究所 组织工程中心 中国医学科学院基础研究所组织工程中心
  • 收稿日期:2008-04-09 修回日期:2008-05-22 出版日期:2009-01-25 发布日期:2009-01-25
  • 通讯作者: 赵春华

Sca1+ mesenchymal stem cells induce hemopoietic stem cells to differentiate into dendritic cells

Yuan CHEN, Xing-xia LIU, Chun-hua ZHAO   

  1. Center of Excellence in Tissue Engineering, IBMS and School of Basic Medicine Center of Excellence in Tissue Engineering, IBMS and School of Basic Medicine Tissue Engineering Center, CAMS
  • Received:2008-04-09 Revised:2008-05-22 Online:2009-01-25 Published:2009-01-25
  • Contact: Chun-hua ZHAO

摘要: 目的 研究Sca1+间充质干细胞对HSC向DC分化的支持作用,并对获得的DC进行形态、表面标志以及功能的鉴定。方法 取健康Balb/c小鼠骨髓,通过MACS系统分离、纯化CD117+造血干细胞;以小鼠Sca1+间充质干细胞做饲养层,诱导CD117+造血干细胞定向分化为树突细胞,显微镜观测形态、流式细胞仪检测表面标志,并通过激光共聚焦显微镜和动物实验检测诱生细胞的功能。结果 培养第10天诱生的DC细胞表面树突较短小,呈毛刺状,高表达CD11b,低表达Ia,对外源颗粒具有吞噬功能,在动物器官移植实验中具有免疫抑制功能。结论 Sca1+间充质干细胞对CD117+HSC向DC分化具有支持作用。

关键词: 造血干细胞, 树突状细胞, 定向分化, Sca1+

Abstract: Objective Study the effect of Sca1+ cells on the HSCs`(hemopoietic stem cells) differentiation to dendritic cells (DCs), and identify the morphology, function and surface markers of the cells. Methods CD117+ HSCs were isolated and purified from the bone marrow of healthy Balb/c mice by magnetic affinity cell sorting. After cell expansion by treatment with the support of Sca1+ cells, the HSCs were induced for directional differentiation into DC-like cells.Studied the surface markers by FACS and function via LSCM and animal experiments. Results After 10 days of culture , we demonstrated that Sca1+ cells induced HSCs to differentiate into a distinct regulatory DC subset with high expression of CD11b but low expression of Ia. They had phagocytotic activity,and can suppressed the GVHD(graft versus host disease) reaction. Conclusion HSCs can differented to dendritic cells with the support of Sca1+ cells.

Key words: hematopoietic stem cell, dendritic cell, directional differitiation, Sca1+

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