基础医学与临床 ›› 2023, Vol. 43 ›› Issue (6): 931-935.doi: 10.16352/j.issn.1001-6325.2023.06.0931

• 研究论文 • 上一篇    下一篇

Asp基因对急性心肌梗死小鼠心功能及Erk1/2表达的影响

吕茂琳, 王存吉, 热洋尼沙·卡的, 高颖*   

  1. 新疆医科大学第一附属医院 综合内三科,新疆 乌鲁木齐 830054
  • 收稿日期:2022-03-21 修回日期:2022-08-17 出版日期:2023-06-05 发布日期:2023-05-31
  • 通讯作者: *sunflowergaoying@163.com
  • 基金资助:
    国家自然科学基金(81660040);新疆医科大学临床医学高峰学科校内配套经费资助项目(33-0104006020801)

Effect of Asp gene on cardiac function and Erk1/2 expression in mice with acute myocardial infarction

LYU Maolin, WANG Cunji, Reyangnisha·KADE, GAO Ying*   

  1. The Third Department of General Internal Medicine, the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054, China
  • Received:2022-03-21 Revised:2022-08-17 Online:2023-06-05 Published:2023-05-31
  • Contact: *sunflowergaoying@163.com

摘要: 目的 探讨促酰化蛋白(Asp)基因对急性心肌梗死(MI)模型小鼠心功能的影响。方法 将C57BL/6野生型小鼠及Asp-/-小鼠各10只分为C57、Asp-/-、C57+MI和Asp-/-+MI组。结扎冠状动脉前降支24 h后行心脏B超检测心脏功能,留取心脏标本前测量小鼠空腹血糖及体质量。伊文思蓝-TTC染色观察存活心肌、缺血心肌和心梗心肌的面积。ELISA法测定白介素8(IL-8)、内皮型一氧化氮合酶(eNOS)及血脂谱的表达。Western blot测定细胞外信号调节蛋白激酶1/2(Erk1/2)和磷酸化p-Erk1/2蛋白的表达。结果 Asp-/-及Asp-/- +MI组的空腹血糖及体质量均分别高于野生型组(P<0.05)。MI后Asp-/-组小鼠的射血分数(EF)及短轴缩短率(FS)较野生型MI组小鼠降低,左心室舒张末内径(LVEDd)及左心室收缩末内径(LVESd)增加(P<0.05)。Asp-/-+MI组缺血区面积明显大于C57+MI组小鼠(P<0.05)。与C57+MI小鼠相比,Asp-/-+MI组小鼠IL-8升高。C57及Asp-/-+MI组小鼠eNOS较术前均降低。Asp-/-+MI组小鼠Erk1/2及p-Erk1/2明显弱于C57+MI组。结论 Asp可能通过MAPK-Erk信号通路改善小鼠心功能、抑制心肌细胞凋亡和减轻小鼠心肌受损。

关键词: 促酰化蛋白, 急性心肌梗死, 细胞外信号调节激酶1/2

Abstract: Objective To investigate the effect of acylation-stimulating protein(Asp) gene on cardiac function in mice with acute myocardial infarction(MI). Methods C57BL/6 wild-type mice and Asp-/- mice were divided into four groups(C57,Asp-/-,C57+MI and Asp-/-+MI) respectively. Twenty-four hours after ligation of the anterior descending coronary artery, heart B-ultrasound was performed to detect the cardiac function, and the fasting blood glucose and body weight of the mice were measured before thoracotomy sampling. Evans blue-TTC staining was used to observe the area of viable myocardium, ischemic myocardium and myocardial infarction. The expression of interleukin-8 (IL-8), endothelial nitric oxide synthase (eNOS) and blood lipid profile was determined by ELISA. The expression of extracellular signal-regulated protein kinase 1/2 (Erk1/2) and phosphorylated p-Erk1/2 protein was determined by Western blot. Results The fasting blood glucose and body weight of Asp-/- control group and Asp-/- myocardial infarction group were higher than those of wild-type group(P<0.05). After myocardial infarction, the EF and FS of the mice in the Asp-/- group were lower than those in the wild-type myocardial infarction group, and the LVEDd and LVESd were increased (P<0.05). The ischemic area of Asp-/- myocardial infarction group was significantly larger than that of wild-type myocardial infarction group(P<0.05). Compared with C57+MI mice, IL-8 in Asp-/- myocardial infarction mice was elevated. The eNOS of wild-type and Asp-/- myocardial infarction mice were lower than those before operation. Erk1/2 and p-Erk1/2 in Asp-/- myocardial infarction group were significantly weaker than those in wild-type myocardial infarction group. Conclusions Asp gene may improve mouse cardiac function, inhibit myocardial apoptosis, and reduce myocardial damage in mice.

Key words: acylation stimulating protein, myocardial infarction, Erk1/2

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