miR-223抑制肺结核大鼠肺部病变

doi:10.16352/j.issn.1001-6325.2022.08.1225

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (8): 1225-1229.doi: 10.16352/j.issn.1001-6325.2022.08.1225

miR-223抑制肺结核大鼠肺部病变

童晓维, 肖韩^*

宜昌市第三人民医院肺病科, 湖北宜昌 443000

收稿日期:2021-01-26 修回日期:2021-10-21 出版日期:2022-08-05 发布日期:2022-08-01
通讯作者: ^*420536658@qq.com
基金资助:
宜昌市卫生科研指导项目(K16-13)

miR-223 inhibits pulmonary lesions of rats with pulmonary tuberculosis

TONG Xiao-wei, XIAO Han^*

Department of Pulmonary Disease, the Third People's Hospital of Yichang City, Yichang 443000, China

Received:2021-01-26 Revised:2021-10-21 Online:2022-08-05 Published:2022-08-01
Contact: ^*420536658@qq.com

摘要/Abstract

摘要： 目的探究miR-223对大鼠肺结核(PT)肺部病变、IFN-γ蛋白表达的作用机制。方法将大鼠分为正常组(NC组)、肺结核大鼠模型组(PT组)和肺结核大鼠模型注射miR-223 mimics组(miR-223组),每组10只。流式细胞仪检测外周血T细胞群,ELISA检测血清中IFN-γ和IL-18水平,并检测结核杆菌菌落数;HE染色观察肺组织病变;免疫组化检测肺组织中IFN-γ蛋白;RT-qPCR检测肺组织中miR-223 mRNA和IFN-γ mRNA表达。结果 PT组外周血T细胞亚群紊乱,CD3⁺%、CD4⁺%水平降低,CD8⁺%水平升高(P＜0.05);miR-223组CD3⁺%、CD4⁺%水平较PT组增加,而CD8⁺%水平降低(P＜0.05)。PT组血清中IFN-γ和IL-18水平较NC组增加(P＜0.05);miR-223组较PT组得到明显抑制(P＜0.05)。PT组结核杆菌菌落数较NC组增多(P＜0.05);miR-223组结核杆菌菌落数较PT组明显减少(P＜0.05)。PT组肺组织总IFN-γ表达较NC组明显升高(P＜0.05);miR-223组较PT组得到明显抑制(P＜0.05)。结论 miR-223可抑制结核分枝杆菌引起的大鼠肺部炎性反应,并且miR-223也有抗结核杆菌和调节机体免疫的作用,同时还能有效抑制肺组织中IFN-γ的表达,进而降低结核病的严重程度。

关键词: miR-223, 肺结核, 肺部病变, IFN-γ蛋白

Abstract: Objective To explore the effect of miR-223 on pulmonary lesions and IFN-γ in rats with pulmonary tuberculosis(PT) mechanism of protein expression. Methods Rats were divided into normal group (NC group), pulmonary tuberculosis rat model group (PT group) and pulmonary tuberculosis rat model transfected with miR-223 mimics group (miR-223 group) with 10 in each group. T cells in peripheral blood was quantified by flow cytometry, IFN-γ in serum was detected by ELISA and IL-18 levels, and the number of Mycobacterium tuberculosis colonies was counted; the pathological changes of lung tissue were micros copied by HE staining; IFN-γ in lung tissue was detected by immunohistochemistry method; RT-qPCR was used to detect miR-223 mRNA and IFN-γ in lung tissue MRNA expression. Results In PT group, the peripheral blood T cell subsets were disordered, the level of CD3⁺,CD4⁺% significantly decreased, and CD8⁺% was increased (P＜0.05); CD3⁺ and CD4⁺ percentage in miR-223 group were higher than those in PT group, while the percentage of CD8⁺ was lower(P＜0.05). Serum IFN-γ and IL-18 in PT group increased(P＜0.05); the number of Mycobacterium tuberculosis colonies in PT group was more than that in NC group (P＜0.05); the colony number of Mycobacterium tuberculosis in miR-223 group was significantly less than that in PT group (P＜0.05). Total IFN-γ level in PT group was higher than that in NC group(P＜0.05) but was significantly inhibited in miR-223 group(P＜0.05). Conclusions miR-223 can inhibit the pulmonary inflammatory response caused by Mycobacterium tuberculosis, and miR-223 showed an effect of anti-tuberculosis bacteria and may regulate host immunity, so and can effectively inhibit the expression of IFN-γ in lung tissue and thus alleviates severity of tuberculosis.

Key words: miR-223, pulmonary tuberculosis, pulmonary lesions, IFN-γ protein

中图分类号:

R521

童晓维, 肖韩. miR-223抑制肺结核大鼠肺部病变[J]. 基础医学与临床, 2022, 42(8): 1225-1229.

TONG Xiao-wei, XIAO Han. miR-223 inhibits pulmonary lesions of rats with pulmonary tuberculosis[J]. Basic & Clinical Medicine, 2022, 42(8): 1225-1229.

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miR-223抑制肺结核大鼠肺部病变

miR-223 inhibits pulmonary lesions of rats with pulmonary tuberculosis

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